BMRB Entry 19427
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19427
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Title: The solution structure of the C-terminal domain of BldD from Streptomyces coelicolor PubMed: 24356916
Deposition date: 2013-08-14 Original release date: 2013-11-26
Authors: Kim, Jeong-Mok; Won, Hyung-Sik; Kang, Sa-Ouk
Citation: Kim, Jeong-Mok; Won, Hyung-Sik; Kang, Sa-Ouk. "The C-terminal domain of the transcriptional regulator BldD from Streptomyces coelicolor A3(2) constitutes a novel fold of winged-helix domains." Proteins ., .-. (2013).
Assembly members:
BldD-CTD, polymer, 89 residues, 9853.219 Da.
Natural source: Common Name: high GC Gram+ Taxonomy ID: 1902 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces coelicolor
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
BldD-CTD: MEPPPKLVLDLERLATVPAE
KAGPLQRYAATIQSQRGDYN
GKVLSIRQDDLRTLAVIYDQ
SPSVLTEQLISWGVLDADAR
RAVASHDEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 609 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BldD-CTD | 1 |
Entities:
Entity 1, BldD-CTD 89 residues - 9853.219 Da.
| 1 | MET | GLU | PRO | PRO | PRO | LYS | LEU | VAL | LEU | ASP | ||||
| 2 | LEU | GLU | ARG | LEU | ALA | THR | VAL | PRO | ALA | GLU | ||||
| 3 | LYS | ALA | GLY | PRO | LEU | GLN | ARG | TYR | ALA | ALA | ||||
| 4 | THR | ILE | GLN | SER | GLN | ARG | GLY | ASP | TYR | ASN | ||||
| 5 | GLY | LYS | VAL | LEU | SER | ILE | ARG | GLN | ASP | ASP | ||||
| 6 | LEU | ARG | THR | LEU | ALA | VAL | ILE | TYR | ASP | GLN | ||||
| 7 | SER | PRO | SER | VAL | LEU | THR | GLU | GLN | LEU | ILE | ||||
| 8 | SER | TRP | GLY | VAL | LEU | ASP | ALA | ASP | ALA | ARG | ||||
| 9 | ARG | ALA | VAL | ALA | SER | HIS | ASP | GLU | LEU |
Samples:
sample_1: BldD-CTD, [U-99% 13C; U-98% 15N], 2 mM; sodium phosphate 40 mM; sodium chloride 150 mM; sodium azide 0.005%; H2O 93%; D2O 7%
Conditions_for_BldD-CTD: ionic strength: 190 mM; pH: 6.8; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 2D 1H-13C HSQC | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D HNCACB | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D HN(CO)CACB | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D HNCO | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D HCCH-TOCSY | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D HCCH-COSY | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D 1H-15N TOCSY | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D 1H-15N NOESY | sample_1 | isotropic | Conditions_for_BldD-CTD |
| 3D 1H-13C NOESY | sample_1 | isotropic | Conditions_for_BldD-CTD |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | GAP50531 |
| EMBL | CAB93369 CQR65077 |
| GB | AAC38279 AEY88188 AGF62344 AIJ16957 AIR97221 |
| REF | NP_625769 WP_003977337 WP_003988955 WP_004990661 WP_006131213 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts