BMRB Entry 19397
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19397
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Title: Backbone 1H and 15N Chemical Shift Assignments for the first domain of FAT10
Deposition date: 2013-07-30 Original release date: 2014-08-25
Authors: Wang, Wei; Lim, Liangzhong; Qin, Haina
Citation: Wang, Wei; Lim, Liangzhong; Qin, Haina; Song, Jianxing. "Structure of FAT10 first domain" Not known ., .-..
Assembly members:
FAT10, polymer, 75 residues, 8676.306 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FAT10: LCVHVRSEEWDLMTFDANPY
DSVLKIKEHVRSKTKVPVQD
QVLLLGSKILKPRRSLSSYG
IDKEKTIHLTLKVVK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 52 |
| 1H chemical shifts | 296 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FAT10 | 1 |
Entities:
Entity 1, FAT10 75 residues - 8676.306 Da.
| 1 | LEU | CYS | VAL | HIS | VAL | ARG | SER | GLU | GLU | TRP | ||||
| 2 | ASP | LEU | MET | THR | PHE | ASP | ALA | ASN | PRO | TYR | ||||
| 3 | ASP | SER | VAL | LEU | LYS | ILE | LYS | GLU | HIS | VAL | ||||
| 4 | ARG | SER | LYS | THR | LYS | VAL | PRO | VAL | GLN | ASP | ||||
| 5 | GLN | VAL | LEU | LEU | LEU | GLY | SER | LYS | ILE | LEU | ||||
| 6 | LYS | PRO | ARG | ARG | SER | LEU | SER | SER | TYR | GLY | ||||
| 7 | ILE | ASP | LYS | GLU | LYS | THR | ILE | HIS | LEU | THR | ||||
| 8 | LEU | LYS | VAL | VAL | LYS |
Samples:
sample_1: sodium phosphate 0.01 mM; DTT 0.01 mM; FAT10, [U-100% 15N], 0.4 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - geometry optimization
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
TOPSPIN, Bruker Biospin - collection
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG34855 |
| EMBL | CAA21458 CAA73200 |
| GB | AAD52982 AAH12472 ABM85550 AIC55799 EAX03201 |
| REF | NP_006389 XP_004043579 |
| SP | O15205 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts