BMRB Entry 19298
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19298
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Title: RBPMS2-Nter PubMed: 25064856
Deposition date: 2013-06-12 Original release date: 2014-10-13
Authors: YANG, Yinshan
Citation: Sagnol, Sebastien; Yang, Yinshan; Bessin, Yannick; Allemand, Frederic; Hapkova, Ilona; Notarnicola, Cecile; Guichou, Jean-Francois; Faure, Sandrine; Labesse, Gilles; de Santa Barbara, Pascal. "Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity" Nucleic Acids Res. 42, 10173-10184 (2014).
Assembly members:
entity, polymer, 93 residues, 10501.271 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MEEEVRTLFVSGLPVDIKPR
ELYLLFRPFKGYEGSLIKLT
ARQPVGFVIFDSRAGAEAAK
NALNGIRFDPENPQTLRLEF
AKANTKMAKSKLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RBPMS2-Nter, 1 | 1 |
| 2 | RBPMS2-Nter, 2 | 1 |
Entities:
Entity 1, RBPMS2-Nter, 1 93 residues - 10501.271 Da.
| 1 | MET | GLU | GLU | GLU | VAL | ARG | THR | LEU | PHE | VAL | ||||
| 2 | SER | GLY | LEU | PRO | VAL | ASP | ILE | LYS | PRO | ARG | ||||
| 3 | GLU | LEU | TYR | LEU | LEU | PHE | ARG | PRO | PHE | LYS | ||||
| 4 | GLY | TYR | GLU | GLY | SER | LEU | ILE | LYS | LEU | THR | ||||
| 5 | ALA | ARG | GLN | PRO | VAL | GLY | PHE | VAL | ILE | PHE | ||||
| 6 | ASP | SER | ARG | ALA | GLY | ALA | GLU | ALA | ALA | LYS | ||||
| 7 | ASN | ALA | LEU | ASN | GLY | ILE | ARG | PHE | ASP | PRO | ||||
| 8 | GLU | ASN | PRO | GLN | THR | LEU | ARG | LEU | GLU | PHE | ||||
| 9 | ALA | LYS | ALA | ASN | THR | LYS | MET | ALA | LYS | SER | ||||
| 10 | LYS | LEU | GLU |
Samples:
sample_1: entity, [U-100% 15N], 0.4-0.8 mM; entity, [U-100% 13C; U-100% 15N], 0.7 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.3; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization
NMR spectrometers:
- Bruker DRX 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAB26834 BAC87172 BAE25995 BAE33785 BAI47052 |
| GB | AAH21788 AAI69038 AAI72373 AAQ73311 ABQ82149 |
| REF | NP_001166897 NP_082306 NP_919248 XP_001174270 XP_002718127 |
| SP | Q6ZRY4 Q8VC52 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts