BMRB Entry 19234
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19234
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Title: 1H, 13C, 15N assignments for P. yoelii Merozoite Surface Protein 1, E28K variant PubMed: 24403012
Deposition date: 2013-05-08 Original release date: 2015-08-04
Authors: Curd, Rachel; Birdsall, Berry; Kadekoppala, Madhusudan; Ogun, Solabomi; Kelly, Geoff; Holder, Anthony
Citation: Curd, Rachel; Birdsall, Berry; Kadekoppala, Madhusudan; Ogun, Solabomi; Kelly, Geoff; Holder, Anthony. "The structure of Plasmodium yoelii merozoite surface protein 119, antibody specificity and implications for malaria vaccine design" Open Biol. 4, 130091-130091 (2014).
Assembly members:
MSP1-E28K, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: Apicomplexans Taxonomy ID: 5861 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium Yoelii
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
MSP1-E28K: GVDPKHVCVDTRDIPKNAGC
FRDDDGTKEWRCLLGYKKGE
GNTCVENNNPTCDINNGGCD
PTASCQNAESTENSKKIICT
CKEPTPNAYYEGVFCSSSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 287 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 606 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MSP1-E28K | 1 |
Entities:
Entity 1, MSP1-E28K 99 residues - Formula weight is not available
| 1 | GLY | VAL | ASP | PRO | LYS | HIS | VAL | CYS | VAL | ASP | ||||
| 2 | THR | ARG | ASP | ILE | PRO | LYS | ASN | ALA | GLY | CYS | ||||
| 3 | PHE | ARG | ASP | ASP | ASP | GLY | THR | LYS | GLU | TRP | ||||
| 4 | ARG | CYS | LEU | LEU | GLY | TYR | LYS | LYS | GLY | GLU | ||||
| 5 | GLY | ASN | THR | CYS | VAL | GLU | ASN | ASN | ASN | PRO | ||||
| 6 | THR | CYS | ASP | ILE | ASN | ASN | GLY | GLY | CYS | ASP | ||||
| 7 | PRO | THR | ALA | SER | CYS | GLN | ASN | ALA | GLU | SER | ||||
| 8 | THR | GLU | ASN | SER | LYS | LYS | ILE | ILE | CYS | THR | ||||
| 9 | CYS | LYS | GLU | PRO | THR | PRO | ASN | ALA | TYR | TYR | ||||
| 10 | GLU | GLY | VAL | PHE | CYS | SER | SER | SER | SER |
Samples:
sample_1: MSP1-E28K, [U-99% 13C; U-99% 15N], 1.0 mM; potassium chloride 50.0 mM; potassium phosphate 25.0 mM; H2O 95%; D2O 5%
sample_conditions_1: pH*: 6.5; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking
TOPSPIN, Bruker Biospin - collection
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 19233 |
| PDB | |
| EMBL | CDU17703 CDZ11970 |
| GB | AAA29702 AAA29762 EAA17822 |
| REF | XP_726257 |
| SP | P13828 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts