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Biological Magnetic Resonance Data Bank


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BMRB Entry 19126

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19126
MolProbity Validation Chart

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Title: Structure of full-length transmembrane domains of human glycine receptor 1 monomer subunit   PubMed: 23994010

Deposition date: 2013-03-28 Original release date: 2013-09-03

Authors: Mowrey, David; Cui, Tanxing; Jia, Yuanyuan; Ma, Dejian; Makhov, Alexander; Zhang, Peijun; Tang, Pei; Xu, Yan

Citation: Mowrey, David; Cui, Tanxing; Jia, Yuanyuan; Ma, Dejian; Makhov, Alexander; Zhang, Peijun; Tang, Pei; Xu, Yan. "Open-Channel Structures of the Human Glycine Receptor 1 Full-Length Transmembrane Domain."  Structure 21, 1897-1904 (2013).

Assembly members:
GlyR, polymer, 150 residues, 17320.490 Da.

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GlyR: MLERQLGYYLIQLYIPSLLI VILSWISFWINLDAAPARVG LGITTVLTLTTQSSGSRASL PKVSYVKAIDIWLAVCLLFV FSALLEYAAVNFVSRQREFG GGGFIQRAKKIDKISRIGFP LAFLIFNLFYWIIYKIVRRE DEFEHHHHHH

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts155
1H chemical shifts707

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GlyR1

Entities:

Entity 1, GlyR 150 residues - 17320.490 Da.

1   METLEUGLUARGGLNLEUGLYTYRTYRLEU
2   ILEGLNLEUTYRILEPROSERLEULEUILE
3   VALILELEUSERTRPILESERPHETRPILE
4   ASNLEUASPALAALAPROALAARGVALGLY
5   LEUGLYILETHRTHRVALLEUTHRLEUTHR
6   THRGLNSERSERGLYSERARGALASERLEU
7   PROLYSVALSERTYRVALLYSALAILEASP
8   ILETRPLEUALAVALCYSLEULEUPHEVAL
9   PHESERALALEULEUGLUTYRALAALAVAL
10   ASNPHEVALSERARGGLNARGGLUPHEGLY
11   GLYGLYGLYPHEILEGLNARGALALYSLYS
12   ILEASPLYSILESERARGILEGLYPHEPRO
13   LEUALAPHELEUILEPHEASNLEUPHETYR
14   TRPILEILETYRLYSILEVALARGARGGLU
15   ASPGLUPHEGLUHISHISHISHISHISHIS

Samples:

sample_1: GlyR 480 uM; H2O 95%; D2O 5%

sample_2: GlyR 480 uM; H2O 95%; D2O 5%

sample_3: GlyR 480 uM; H2O 95%; D2O 5%

sample_4: GlyR 480 uM; H2O 95%; D2O 5%

sample_5: GlyR 480 uM; H2O 95%; D2O 5%

sample_6: GlyR 480 uM; H2O 95%; D2O 5%

sample_7: GlyR 480 uM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

CYANA v3.0 -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

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Download simulated HSQC data in one of the following formats:
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