BMRB Entry 19034
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19034
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Title: NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Deposition date: 2013-02-12 Original release date: 2013-03-21
Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt
Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2" Not known ., .-..
Assembly members:
U2AF, polymer, 105 residues, 12033.749 Da.
Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
U2AF: GGHPTEVLCLMNMVLPEELL
DDEEYEEIVEDVRDECSKYG
LVKSIEIPRPVDGVEVPGCG
KIFVEFTSVFDCQKAMQGLT
GRKFANRVVVTKYCDPDSYH
RRDFW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 367 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 721 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | U2AF | 1 |
Entities:
Entity 1, U2AF 105 residues - 12033.749 Da.
| 1 | GLY | GLY | HIS | PRO | THR | GLU | VAL | LEU | CYS | LEU | ||||
| 2 | MET | ASN | MET | VAL | LEU | PRO | GLU | GLU | LEU | LEU | ||||
| 3 | ASP | ASP | GLU | GLU | TYR | GLU | GLU | ILE | VAL | GLU | ||||
| 4 | ASP | VAL | ARG | ASP | GLU | CYS | SER | LYS | TYR | GLY | ||||
| 5 | LEU | VAL | LYS | SER | ILE | GLU | ILE | PRO | ARG | PRO | ||||
| 6 | VAL | ASP | GLY | VAL | GLU | VAL | PRO | GLY | CYS | GLY | ||||
| 7 | LYS | ILE | PHE | VAL | GLU | PHE | THR | SER | VAL | PHE | ||||
| 8 | ASP | CYS | GLN | LYS | ALA | MET | GLN | GLY | LEU | THR | ||||
| 9 | GLY | ARG | LYS | PHE | ALA | ASN | ARG | VAL | VAL | VAL | ||||
| 10 | THR | LYS | TYR | CYS | ASP | PRO | ASP | SER | TYR | HIS | ||||
| 11 | ARG | ARG | ASP | PHE | TRP |
Samples:
sample_1: U2AF, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 5 mM; DTT 8 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1298 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 4D-HACANH-APSY | sample_1 | isotropic | sample_conditions_1 |
| 5D-CBCACONH-APSY | sample_1 | isotropic | sample_conditions_1 |
| 5D-HACACONH-APSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, G ntert P. - structure solution
OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
TOPSPIN v3.1, Bruker Biospin - collection, processing
CARA, Keller and Wuthrich - chemical shift assignment, data analysis
j-UNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC37309 BAG70075 BAG70201 BAI45669 |
| EMBL | CAA45409 CAA45874 CAA45875 |
| GB | AAH07487 AAH08740 AAH30574 AAH43071 AAH89996 |
| PRF | 1805352A |
| REF | NP_001012496 NP_001068804 NP_001192160 NP_001302733 NP_009210 |
| SP | P26368 P26369 |
| TPG | DAA19368 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts