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Biological Magnetic Resonance Data Bank


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BMRB Entry 19013

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19013
MolProbity Validation Chart

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Title: Solution structure of the RRM domain of the hypothetical protein CAGL0M09691g from Candida glabrata

Deposition date: 2013-02-07 Original release date: 2013-03-06

Authors: Harris, R.; Hillerich, B.; Ahmed, M.; Bonanno, J.; Chamala, S.; Evans, B.; Lafleur, J.; Hammonds, J.; Washington, E.; Stead, M.; Love, J.; Attonito, J.; Seidel, R.; Chook, Y.; Rout, M.; Girvin, M.; Almo, S.; Harris, R.; Hillerich, B.; Ahmed, M.; Bonanno, J.; Chamala, S.; Evans, B.; Lafleur, J.; Washington, E.; Stead, M.; Love, J.; Attonito, J.; Patel, H.; Seidel, R.; Chook, Y.; Rout, M.; Girvin, M.; Almo, S.

Citation: Harris, R.; Hillerich, B.; Ahmed, M.; Bonanno, J.; Chamala, S.; Evans, B.; Lafleur, J.; Hammonds, J.; Washington, E.; Stead, M.; Love, J.; Attonito, J.; Patel, H.; Seidel, R.; Chook, Y.; Rout, M.; Girvin, M.; Almo, S.. "Solution structure of the RRM domain of the hypothetical protein CAGL0M09691g from Candida glabrata"  To be published ., .-..

Assembly members:
RRM_domain_of_the_hypothetical_protein, polymer, 124 residues, 14227.990 Da.

Natural source:   Common Name: ascomycetes   Taxonomy ID: 5478   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Candida glabrata

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RRM_domain_of_the_hypothetical_protein: MSLGSESETGNAVVVFGYRE AITKQILAYFAQFGEILEDL ESELGDTETMRTPGYFFQQA PNRRRISREHGRTWTKLTYA NHSSYLRALREHGTIYCGAA IGCVPYKHELISELSREGHH HHHH

Data sets:
Data typeCount
13C chemical shifts532
15N chemical shifts131
1H chemical shifts816

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM domain of the hypothetical protein1

Entities:

Entity 1, RRM domain of the hypothetical protein 124 residues - 14227.990 Da.

expressed sequence start-stop 171-283 N-term cloning artifact: MSL C-term cloning artifact: EGHHHHHH

1   METSERLEUGLYSERGLUSERGLUTHRGLY
2   ASNALAVALVALVALPHEGLYTYRARGGLU
3   ALAILETHRLYSGLNILELEUALATYRPHE
4   ALAGLNPHEGLYGLUILELEUGLUASPLEU
5   GLUSERGLULEUGLYASPTHRGLUTHRMET
6   ARGTHRPROGLYTYRPHEPHEGLNGLNALA
7   PROASNARGARGARGILESERARGGLUHIS
8   GLYARGTHRTRPTHRLYSLEUTHRTYRALA
9   ASNHISSERSERTYRLEUARGALALEUARG
10   GLUHISGLYTHRILETYRCYSGLYALAALA
11   ILEGLYCYSVALPROTYRLYSHISGLULEU
12   ILESERGLULEUSERARGGLUGLYHISHIS
13   HISHISHISHIS

Samples:

sample_1: RRM domain of the hypothetical protein, [U-100% 13C; U-100% 15N], 1.0 mM; sodium acetate 10 mM; sodium chloride 100 mM; DTT 0.5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: RRM domain of the hypothetical protein, [U-100% 13C; U-100% 15N], 1.0 mM; sodium acetate 10 mM; sodium chloride 100 mM; DTT 0.5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 110 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_1isotropicsample_conditions_1
15N NOESY-HSQCsample_1isotropicsample_conditions_1
13C HSQCsample_2isotropicsample_conditions_1
aromatic 13C HSQCsample_2isotropicsample_conditions_1
13C NOESY-HSQCsample_2isotropicsample_conditions_1
13C aromatic NOESY-HSQCsample_2isotropicsample_conditions_1
HNCOsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
HNCAsample_1isotropicsample_conditions_1
HNCOCAsample_1isotropicsample_conditions_1
HNCACBsample_1isotropicsample_conditions_1
CBCACONHsample_1isotropicsample_conditions_1

Software:

CNS v1.21, Brunger A. T. et.al. - refinement

VNMRJ v2.2D, Varian - collection

TOPSPIN v2.1, Bruker Biospin - collection

MDDNMR v2.2, (MDDNMR) Orekhov, Jaravine, Kazimierczuk - collection, processing

CCPN_Analysis v2.2, CCPN - data analysis

ARIA v2.3, Linge, O, . - data analysis

X-PLOR NIH v2.32, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian Inova 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP Q6FJ27
PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts