BMRB Entry 18882
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18882
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Title: TICAM-2 TIR domain PubMed: 24255114
Deposition date: 2012-12-07 Original release date: 2014-01-13
Authors: Enokizono, Yoshiaki; Kumeta, Hiroyuki; Funami, Kenji; Horiuchi, Masataka; Sarmiento, Joy; Yamashita, Kazuo; Standley, Daron; Matsumoto, Misako; Seya, Tsukasa; Inagaki, Fuyuhiko
Citation: Enokizono, Yoshiaki; Kumeta, Hiroyuki; Funami, Kenji; Horiuchi, Masataka; Sarmiento, Joy; Yamashita, Kazuo; Standley, Daron; Matsumoto, Misako; Seya, Tsukasa; Inagaki, Fuyuhiko. "Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling" Proc. Natl. Acad. Sci. U. S. A. 110, 19908-19913 (2013).
Assembly members:
TICAM-2_TIR, polymer, 166 residues, 19386.037 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TICAM-2_TIR: GPLGSEEVFLKFVILHAEDD
TDEALRVQNLLQDDFGIKPG
IIFAEMPHGRQHLQNLDDAV
NGSAWTILLLTENFLRDTWC
NFQFYTSLMNSVNRQHKYNS
VIPMRPLNNPLPRERTPFAL
QTINALEEESRGFPTQVERI
FQESVYKTQQTIWKETRNMV
QRQFIA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 696 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 1193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TICAM-2 TIR domain | 1 |
Entities:
Entity 1, TICAM-2 TIR domain 166 residues - 19386.037 Da.
| 1 | GLY | PRO | LEU | GLY | SER | GLU | GLU | VAL | PHE | LEU | ||||
| 2 | LYS | PHE | VAL | ILE | LEU | HIS | ALA | GLU | ASP | ASP | ||||
| 3 | THR | ASP | GLU | ALA | LEU | ARG | VAL | GLN | ASN | LEU | ||||
| 4 | LEU | GLN | ASP | ASP | PHE | GLY | ILE | LYS | PRO | GLY | ||||
| 5 | ILE | ILE | PHE | ALA | GLU | MET | PRO | HIS | GLY | ARG | ||||
| 6 | GLN | HIS | LEU | GLN | ASN | LEU | ASP | ASP | ALA | VAL | ||||
| 7 | ASN | GLY | SER | ALA | TRP | THR | ILE | LEU | LEU | LEU | ||||
| 8 | THR | GLU | ASN | PHE | LEU | ARG | ASP | THR | TRP | CYS | ||||
| 9 | ASN | PHE | GLN | PHE | TYR | THR | SER | LEU | MET | ASN | ||||
| 10 | SER | VAL | ASN | ARG | GLN | HIS | LYS | TYR | ASN | SER | ||||
| 11 | VAL | ILE | PRO | MET | ARG | PRO | LEU | ASN | ASN | PRO | ||||
| 12 | LEU | PRO | ARG | GLU | ARG | THR | PRO | PHE | ALA | LEU | ||||
| 13 | GLN | THR | ILE | ASN | ALA | LEU | GLU | GLU | GLU | SER | ||||
| 14 | ARG | GLY | PHE | PRO | THR | GLN | VAL | GLU | ARG | ILE | ||||
| 15 | PHE | GLN | GLU | SER | VAL | TYR | LYS | THR | GLN | GLN | ||||
| 16 | THR | ILE | TRP | LYS | GLU | THR | ARG | ASN | MET | VAL | ||||
| 17 | GLN | ARG | GLN | PHE | ILE | ALA |
Samples:
CN: TICAM-2 TIR, [U-99% 13C; U-99% 15N], 0.5 mM; HEPES-NaOH 50 mM; DTT 10 mM; glycerol, [U-100% 2H], 5%; DSS 0.02 mg/mL
sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | CN | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | CN | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | CN | isotropic | sample_conditions_1 |
| 3D HNCO | CN | isotropic | sample_conditions_1 |
| 3D HNCA | CN | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | CN | isotropic | sample_conditions_1 |
| 3D HNCACB | CN | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | CN | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | CN | isotropic | sample_conditions_1 |
| 3D HN(CA)HA | CN | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | CN | isotropic | sample_conditions_1 |
| 3D C(CO)NH | CN | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | CN | isotropic | sample_conditions_1 |
| 3D 1H-15N-NOESY | CN | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | CN | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | CN | isotropic | sample_conditions_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v2007.068.09.07, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.113, Goddard - chemical shift assignment, peak picking, refinement
TALOS v2007.068.09.07, Cornilescu, Delaglio and Bax - data analysis
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB21882 BAC77375 BAC98397 BAC98399 BAE88603 |
| GB | AAI09266 AAI09267 AAO74498 AAP81748 AAQ97430 |
| REF | NP_001157940 NP_001182133 NP_001182430 NP_001191270 NP_001191272 |
| SP | Q86XR7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts