BMRB Entry 18876
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18876
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Complex of MDM2(3-109) and p73 TAD(10-25) PubMed: 25591003
Deposition date: 2012-12-05 Original release date: 2015-06-01
Authors: Shin, Jae-Sun; Ha, Jihyang; Chi, Seung-Wook
Citation: Shin, Jae-Sun; Ha, Ji-Hyang; Lee, Dong-Hwa; Ryu, Kyoung-Seok; Bae, Kwang-Hee; Park, Byoung-Chul; Park, Sung-Goo; Yi, Gwan-Su; Chi, Seung-Wook. "Structural convergence of unstructured p53 family transactivation domains in MDM2 recognition" Cell Cycle 14, 533-543 (2015).
Assembly members:
entity_1, polymer, 107 residues, 12275.353 Da.
entity_2, polymer, 16 residues, 1791.864 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: NTNMSVPTDGAVTTSQIPAS
EQETLVRPKPLLLKLLKSVG
AQKDTYTMKEVLFYLGQYIM
TKRLYDEKQQHIVYCSNDLL
GDLFGVPSFSVKEHRKIYTM
IYRNLVV
entity_2: DGGTTFEHLWSSLEPD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 384 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 762 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MDM2(3-109) | 1 |
| 2 | p73_TAD(10-25) | 2 |
Entities:
Entity 1, MDM2(3-109) 107 residues - 12275.353 Da.
| 1 | ASN | THR | ASN | MET | SER | VAL | PRO | THR | ASP | GLY | ||||
| 2 | ALA | VAL | THR | THR | SER | GLN | ILE | PRO | ALA | SER | ||||
| 3 | GLU | GLN | GLU | THR | LEU | VAL | ARG | PRO | LYS | PRO | ||||
| 4 | LEU | LEU | LEU | LYS | LEU | LEU | LYS | SER | VAL | GLY | ||||
| 5 | ALA | GLN | LYS | ASP | THR | TYR | THR | MET | LYS | GLU | ||||
| 6 | VAL | LEU | PHE | TYR | LEU | GLY | GLN | TYR | ILE | MET | ||||
| 7 | THR | LYS | ARG | LEU | TYR | ASP | GLU | LYS | GLN | GLN | ||||
| 8 | HIS | ILE | VAL | TYR | CYS | SER | ASN | ASP | LEU | LEU | ||||
| 9 | GLY | ASP | LEU | PHE | GLY | VAL | PRO | SER | PHE | SER | ||||
| 10 | VAL | LYS | GLU | HIS | ARG | LYS | ILE | TYR | THR | MET | ||||
| 11 | ILE | TYR | ARG | ASN | LEU | VAL | VAL |
Entity 2, p73_TAD(10-25) 16 residues - 1791.864 Da.
| 1 | ASP | GLY | GLY | THR | THR | PHE | GLU | HIS | LEU | TRP | ||||
| 2 | SER | SER | LEU | GLU | PRO | ASP |
Samples:
MDM2-p73TAD: MDM2(3-109), [U-95% 13C; U-95% 15N], 0.9 mM; p73 TAD(10-25), [U-95% 13C; U-95% 15N], 0.9 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D HNCO | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D HNCACB | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | MDM2-p73TAD | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | MDM2-p73TAD | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.26, Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Guntert, Mumenthaler and Wuthrich, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 11569 18755 2410 |
| PDB | |
| DBJ | BAB11975 BAF83030 BAJ17752 |
| EMBL | CAA78055 CAD23251 CAD36959 CAD79457 CAH89564 |
| GB | AAA60568 AAF28866 AAG42840 AAI42265 AAI48523 |
| PRF | 1814460A |
| REF | NP_001003103 NP_001092577 NP_001124685 NP_001138809 NP_001138811 |
| SP | P56951 Q00987 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts