BMRB Entry 18872

Name: ID3 stem
Published: 2012-12-13

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PDB ID: 2m1o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18872
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: ID3 stem

Deposition date: 2012-12-03 Original release date: 2012-12-13

Authors: Popovic, Milena; Greenbaum, Nancy

Citation: Popovic, Milena; Greenbaum, Nancy. "ID3 stem" Not known ., .-..

Assembly members:
RNA_GGGUGUA, polymer, 7 residues, Formula weight is not available
RNA_AGCACCC, polymer, 7 residues, Formula weight is not available

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: enzymatic semisynthesis Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
RNA_GGGUGUA: GGGUGUA
RNA_AGCACCC: AGCACCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	59
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GGGUGUA	1
2	AGCACCC	2

Entities:

Entity 1, GGGUGUA 7 residues - Formula weight is not available

1

G

U

G

U

A

Entity 2, AGCACCC 7 residues - Formula weight is not available

1

A

G

C

A

C

Samples:

sample_1: RNA_GGGUGUA 1 mM; RNA_AGCACCC 1 mM; sodium chloride 80 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.4; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CCPN, CCPN - chemical shift assignment

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz