BMRB Entry 18870
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18870
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Title: Solution structure of the dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum)
Deposition date: 2012-12-03 Original release date: 2013-12-02
Authors: Kovermann, Michael; Dhurvas Chandrasekaran, Dinesh; Gopalswamy, Mohanraj; Abel, Steffen; Balbach, Jochen
Citation: Dhurvas Chandrasekaran, Dinesh; Kovermann, Michael; Gopalswamy, Mohanraj; Balbach, Jochen; Abel, Steffen. "Solution structure of the dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum)" Not known ., .-..
Assembly members:
PsIAA4_DIII/IV, polymer, 107 residues, 11938.721 Da.
Natural source: Common Name: garden pea Taxonomy ID: 3888 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pisum sativum
Experimental source: Production method: recombinant technology Host organism: Pisum sativum
Entity Sequences (FASTA):
PsIAA4_DIII/IV: HEADVGGIFVKVSMDGAPYL
RKIDLRVYGGYSELLKALET
MFKLTIGEYSEREGYKGSEY
APTYEDKDGDWMLVGDVPWD
MFVTSCKRLRIMKGTEAKGL
GCGVGSH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 399 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 629 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PsIAA4_DIII/IV | 1 |
Entities:
Entity 1, PsIAA4_DIII/IV 107 residues - 11938.721 Da.
86-189 aa
| 1 | HIS | GLU | ALA | ASP | VAL | GLY | GLY | ILE | PHE | VAL | ||||
| 2 | LYS | VAL | SER | MET | ASP | GLY | ALA | PRO | TYR | LEU | ||||
| 3 | ARG | LYS | ILE | ASP | LEU | ARG | VAL | TYR | GLY | GLY | ||||
| 4 | TYR | SER | GLU | LEU | LEU | LYS | ALA | LEU | GLU | THR | ||||
| 5 | MET | PHE | LYS | LEU | THR | ILE | GLY | GLU | TYR | SER | ||||
| 6 | GLU | ARG | GLU | GLY | TYR | LYS | GLY | SER | GLU | TYR | ||||
| 7 | ALA | PRO | THR | TYR | GLU | ASP | LYS | ASP | GLY | ASP | ||||
| 8 | TRP | MET | LEU | VAL | GLY | ASP | VAL | PRO | TRP | ASP | ||||
| 9 | MET | PHE | VAL | THR | SER | CYS | LYS | ARG | LEU | ARG | ||||
| 10 | ILE | MET | LYS | GLY | THR | GLU | ALA | LYS | GLY | LEU | ||||
| 11 | GLY | CYS | GLY | VAL | GLY | SER | HIS |
Samples:
sample_1: PsIAA4_DIII/IV, [U-98% 13C; U-98% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; magnesium chloride 2 mM; DTT 1 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0.112 M; pH: 2.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.3, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
ARIA, Linge, O, . - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts