BMRB Entry 18865
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18865
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Title: Ovine Doppel Signal peptide (1-30) PubMed: 23973967
Deposition date: 2012-11-28 Original release date: 2013-10-14
Authors: Pimenta, Jorge; Viegas, Aldino; Sardinha, Joao; Santos, Ana; Cantante, Catia; Dias, Fernando; Soares, Rui; Cabrita, Eurico; Fontes, Carlos; Prates, Jose; Pereira, Rosa
Citation: Pimenta, Jorge; Viegas, Aldino; Sardinha, Joao; Martins, Ivo; Cabrita, Eurico; Fontes, Carlos; Prates, Jose; Pereira, Rosa. "NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein" Peptides 49, 32-40 (2013).
Assembly members:
OvDpl(1-30), polymer, 30 residues, 3321.144 Da.
Natural source: Common Name: sheep Taxonomy ID: 9940 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ovis aries
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
OvDpl(1-30): MRKHLGGCWLAIVCVLLFSQ
LSSVKARGIK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OvDpl(1-30) | 1 |
Entities:
Entity 1, OvDpl(1-30) 30 residues - 3321.144 Da.
| 1 | MET | ARG | LYS | HIS | LEU | GLY | GLY | CYS | TRP | LEU | |
| 2 | ALA | ILE | VAL | CYS | VAL | LEU | LEU | PHE | SER | GLN | |
| 3 | LEU | SER | SER | VAL | LYS | ALA | ARG | GLY | ILE | LYS |
Samples:
sample_1: DHPC 100 mM; OvDpl(1-30) 3 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER v12, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAC22340 |
| GB | AAK08630 AAK77164 AAO44923 ABN79633 ABN79636 |
| REF | NP_001009261 XP_004276488 XP_004324935 XP_005688215 XP_005974367 |
| SP | Q9GJY2 |
| TPE | CAL59571 |