BMRB Entry 18680
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18680
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp PubMed: 23506337
Deposition date: 2012-08-27 Original release date: 2013-08-05
Authors: Lee, Juho; Jeong, Ki-Woong; Kim, Yangmee
Citation: Lee, Juho; Jeong, Ki-Woong; Jin, Bonghwan; Ryu, Kyoung-Seok; Kim, Eun-Hee; Ahn, Joong-Hoon; Kim, Yangmee. "Structural and Dynamic Features of Cold-Shock Proteins of Listeriamonocytogenes, a Psychrophilic Bacterium" Biochemistry 52, 2492-2504 (2013).
Assembly members:
LmCsp, polymer, 66 residues, 7272.949 Da.
Natural source: Common Name: Firmicutes Taxonomy ID: 1639 Superkingdom: Bacteria Kingdom: not available Genus/species: Listeria monocytogenes
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LmCsp: MEQGTVKWFNAEKGFGFIER
ENGDDVFVHFSAIQGDGFKS
LDEGQAVTFDVEEGQRGPQA
ANVQKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 393 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LmCsp | 1 |
Entities:
Entity 1, LmCsp 66 residues - 7272.949 Da.
| 1 | MET | GLU | GLN | GLY | THR | VAL | LYS | TRP | PHE | ASN | ||||
| 2 | ALA | GLU | LYS | GLY | PHE | GLY | PHE | ILE | GLU | ARG | ||||
| 3 | GLU | ASN | GLY | ASP | ASP | VAL | PHE | VAL | HIS | PHE | ||||
| 4 | SER | ALA | ILE | GLN | GLY | ASP | GLY | PHE | LYS | SER | ||||
| 5 | LEU | ASP | GLU | GLY | GLN | ALA | VAL | THR | PHE | ASP | ||||
| 6 | VAL | GLU | GLU | GLY | GLN | ARG | GLY | PRO | GLN | ALA | ||||
| 7 | ALA | ASN | VAL | GLN | LYS | ALA |
Samples:
sample_1: LmCsp, [U-13C; U-15N], 0.8 mM; potassium phosphate 50 mM; EDTA 0.1 mM; potassium chloride 100 mM; DSS 0.004 v/v; H2O 90%; D2O 10%
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Brunger, Adams, Clore, Gros, Nilges and Read, Guntert, Mumenthaler and Wuthrich - chemical shift calculation, refinement
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 18679 |
| PDB | |
| EMBL | CAA62903 CAC20630 CAC96632 CAC99442 CAK20797 |
| GB | AAC80246 AAT04156 ACK39554 ADB68252 ADB71297 |
| REF | NP_464889 WP_003719639 WP_003756020 WP_012985572 |
| SP | P0A355 P0A356 |
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