BMRB Entry 18625
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18625
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Title: NMR Structure of the Self-Complementary 10 mer DNA Oligonucleotide 5'-GGATATATCC-3'. PubMed: 23355266
Deposition date: 2012-07-31 Original release date: 2013-01-22
Authors: Rettig, Michael; Germann, Markus; Wilson, W. David; Wang, Shuo
Citation: Rettig, Michael; Germann, Markus; Wang, Shuo; Wilson, W. David. "Molecular basis for sequence-dependent induced DNA bending." Chembiochem 14, 323-331 (2013).
Assembly members:
DNA_(5'-D(*GP*GP*AP*TP*AP*TP*AP*TP*CP*C)-3')_, polymer, 10 residues, 3044.042 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
DNA_(5'-D(*GP*GP*AP*TP*AP*TP*AP*TP*CP*C)-3')_: GGATATATCC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 200 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GGATATATCC, 1 | 1 |
| 2 | GGATATATCC, 2 | 1 |
Entities:
Entity 1, GGATATATCC, 1 10 residues - 3044.042 Da.
| 1 | DG | DG | DA | DT | DA | DT | DA | DT | DC | DC |
Samples:
sample_1_D2O: GGATATATCC 0.9 mM
sample_1_H2O: GGATATATCC 0.9 mM
sample_1_Pf1: GGATATATCC 1.2 mM; Pf1 phage 11 mg/mL
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1_D2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1_H2O | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1_D2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1_Pf1 | anisotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1_Pf1 | anisotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1_Pf1 | anisotropic | sample_conditions_1 |
Software:
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz