BMRB Entry 18405
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18405
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Title: Structure of Sviceucin, an type I lasso peptide from Streptomyces sviceus
Deposition date: 2012-04-18 Original release date: 2012-08-30
Authors: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie
Citation: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie. "Structure and biosynthesis of Sviceucin, an type I lasso peptide from Streptomyces sviceus" . ., .-..
Assembly members:
entity, polymer, 20 residues, 2106.426 Da.
Natural source: Common Name: high GC Gram+ Taxonomy ID: 285530 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces sviceus
Experimental source: Production method: recombinant technology Host organism: Streptomyces coelicolor
Entity Sequences (FASTA):
entity: CVWGGDCTDFLGCGTAWICV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 59 |
| 1H chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sviceucin | 1 |
Entities:
Entity 1, Sviceucin 20 residues - 2106.426 Da.
| 1 | CYS | VAL | TRP | GLY | GLY | ASP | CYS | THR | ASP | PHE | |
| 2 | LEU | GLY | CYS | GLY | THR | ALA | TRP | ILE | CYS | VAL |
Samples:
sample_1: Sviceucin 2.4 mM; Methanol 2.4 mM
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, CCPN, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - data analysis, processing, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz