BMRB Entry 18336

Name: Structure of the RNA claw of the DNA packaging motor of bacteriophage 29
Published: 2012-07-30

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PDB ID: 2lqz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18336
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of the RNA claw of the DNA packaging motor of bacteriophage 29 PubMed: 22879380

Deposition date: 2012-03-19 Original release date: 2012-07-30

Authors: Harjes, Elena; Matsuo, Hiroshi; Kitamura, Aya

Citation: Harjes, Elena; Kitamura, Aya; Zhao, Wei; Morais, Marc; Jardine, Paul; Grimes, Shelley; Matsuo, Hiroshi. "Structure of the RNA claw of the DNA packaging motor of bacteriophage 29." Nucleic Acids Res. 40, 9953-9963 (2012).

Assembly members:
RNA_(27-MER), polymer, 27 residues, 8586.186 Da.

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: Phi 29

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_(27-MER): GGACUUCCAUUGCUUCGGCA AAAGUCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	69
15N chemical shifts	9
1H chemical shifts	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA (27-MER)	1

Entities:

Entity 1, RNA (27-MER) 27 residues - 8586.186 Da.

1

G

A

C

U

C

A

U

2

U

G

C

U

C

G

C

A

3

A

G

U

C

Samples:

sample_1: RNA (27-MER), [U-100% 13C; U-100% 15N], 300 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	anisotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	anisotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	anisotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz
Varian INOVA 900 MHz