BMRB Entry 17990
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17990
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Title: STRUCTURES AND INTERACTIONS ANALYSES OF THE INTEGRIN ALPHA-M BETA-2 CYTOPLASMIC TAILS PubMed: 22052909
Deposition date: 2011-10-11 Original release date: 2011-11-09
Authors: Chua, Geok Lin; Tang, Xiao Yan; Amalraj, Monalisa; Bhattacharjya, Surajit; Tan, Suet Mien
Citation: Chua, Geok-Lin; Tang, Xiao-Yan; Amalraj, Monalisa; Tan, Suet-Mien; Bhattacharjya, Surajit. "Structures and interaction analyses of integrin M2 cytoplasmic tails." J. Biol. Chem. 286, 43842-43854 (2011).
Assembly members:
entity, polymer, 24 residues, 2703.118 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: KLGFFKRQYKDMMSEGGPPG
AEPQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 22 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MYRISTOYLATED ALPHA M | 1 |
Entities:
Entity 1, MYRISTOYLATED ALPHA M 24 residues - 2703.118 Da.
INTEGRIN MAC-1 ALPHA CYTOPLASMIC TAIL
| 1 | LYS | LEU | GLY | PHE | PHE | LYS | ARG | GLN | TYR | LYS | ||||
| 2 | ASP | MET | MET | SER | GLU | GLY | GLY | PRO | PRO | GLY | ||||
| 3 | ALA | GLU | PRO | GLN |
Samples:
MYR-ALPHA-M: MAC-1 0.7 mM; deuterated Dodecylphosphocholine, [U-99% 2H], 200 mM; sodium phosphate 10 mM; H2O 90%; D2O, [U-99% 2H], 10%
MYR-ALPHA-M_D2O: MAC-1 0.7 mM; deuterated Dodecylphosphocholine, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-99% 2H], 100%
sample_conditions_1: ionic strength: 10 mM; pH: 5.6; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | MYR-ALPHA-M | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | MYR-ALPHA-M | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | MYR-ALPHA-M_D2O | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | MYR-ALPHA-M_D2O | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
TOPSPIN v1.3, Bruker Biospin - processing
SPARKY v3.11, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE91421 BAG10590 BAG54538 |
| GB | AAA59491 AAA59544 AAA59903 AAB24821 AAH96346 |
| REF | NP_000623 NP_001139280 NP_001181863 XP_003280535 XP_004692013 |
| SP | P11215 |