BMRB Entry 17772
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17772
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Title: Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6) PubMed: 22130672
Deposition date: 2011-07-08 Original release date: 2011-12-06
Authors: Kappo, M.; Ab, E.; Atkinson, R.; Faro, A.; Muleya, V.; Mulaudzi, T.; Poole, J.; Mckenzie, J.; PUGH, DAVID
Citation: Kappo, Mautin; Ab, Eiso; Hassem, Faqeer; Atkinson, R. Andrew; Faro, Andrew; Muleya, Victor; Mulaudzi, Takalani; Poole, John; McKenzie, Jean; Chibi, Moredreck; Moolman-Smook, Joanna; Rees, D. Jasper G; Pugh, David. "Solution Structure of RING Finger-like Domain of Retinoblastoma-binding Protein-6 (RBBP6) Suggests It Functions as a U-box." J. Biol. Chem. 287, 7146-7158 (2012).
Assembly members:
E3_UBIQUITIN-PROTEIN_LIGASE_RBBP6, polymer, 92 residues, 10214.4517 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E3_UBIQUITIN-PROTEIN_LIGASE_RBBP6: GPLGSEDDPIPDELLCLICK
DIMTDAVVIPCCGNSYCDEC
IRTALLESDEHTCPTCHQND
VSPDALIANKFLRQAVNNFK
NETGYTKRLRKQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 300 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 631 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E3 UBIQUITIN-PROTEIN LIGASE RBBP6 1 | 1 |
| 2 | E3 UBIQUITIN-PROTEIN LIGASE RBBP6 2 | 1 |
| 3 | Zinc ion, 1 | 2 |
| 4 | Zinc ion, 2 | 2 |
Entities:
Entity 1, E3 UBIQUITIN-PROTEIN LIGASE RBBP6 1 92 residues - 10214.4517 Da.
| 1 | GLY | PRO | LEU | GLY | SER | GLU | ASP | ASP | PRO | ILE | ||||
| 2 | PRO | ASP | GLU | LEU | LEU | CYS | LEU | ILE | CYS | LYS | ||||
| 3 | ASP | ILE | MET | THR | ASP | ALA | VAL | VAL | ILE | PRO | ||||
| 4 | CYS | CYS | GLY | ASN | SER | TYR | CYS | ASP | GLU | CYS | ||||
| 5 | ILE | ARG | THR | ALA | LEU | LEU | GLU | SER | ASP | GLU | ||||
| 6 | HIS | THR | CYS | PRO | THR | CYS | HIS | GLN | ASN | ASP | ||||
| 7 | VAL | SER | PRO | ASP | ALA | LEU | ILE | ALA | ASN | LYS | ||||
| 8 | PHE | LEU | ARG | GLN | ALA | VAL | ASN | ASN | PHE | LYS | ||||
| 9 | ASN | GLU | THR | GLY | TYR | THR | LYS | ARG | LEU | ARG | ||||
| 10 | LYS | GLN |
Entity 2, Zinc ion, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: E3 UBIQUITIN-PROTEIN LIGASE RBBP6, [U-13C; U-15N], 0.7 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC-NOESY 13C-HSQC-NOESY | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS v2.1, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment
CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
NMRView vany, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
| UNP | RBBP6_HUMAN |
| BMRB | 11159 17466 |
| PDB | |
| DBJ | BAC77636 BAC77637 BAG64162 |
| EMBL | CAA59445 |
| GB | AAB49620 AAC72432 AAH63524 AAI72357 AAL05625 |
| REF | NP_001291483 NP_008841 NP_035377 NP_061173 XP_001076339 |
| SP | P97868 Q7Z6E9 |
| TPG | DAA15466 |
Download simulated HSQC data in one of the following formats:
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