BMRB Entry 17699
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17699
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Title: SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL PubMed: 22359612
Deposition date: 2011-06-13 Original release date: 2012-03-02
Authors: Ng, C.; Kuchel, P.; Vandenberg, J.
Citation: Ng, Chai Ann; Perry, Matthew; Tan, Peter; Hill, Adam; Kuchel, Philip; Vandenberg, Jamie. "The s4-s5 linker acts as a signal integrator for HERG k channel activation and deactivation gating." PLoS ONE 7, e31640-. (2012).
Assembly members:
S4S5_Linker, polymer, 20 residues, 2330.752 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
S4S5_Linker: LVRVARKLDRYSEYGAAVLF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL | 1 |
Entities:
Entity 1, SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL 20 residues - 2330.752 Da.
| 1 | LEU | VAL | ARG | VAL | ALA | ARG | LYS | LEU | ASP | ARG | |
| 2 | TYR | SER | GLU | TYR | GLY | ALA | ALA | VAL | LEU | PHE |
Samples:
sample: DPC, {U-2H], 100 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | isotropic | sample_conditions_1 |
| 2D TOCSY | sample | isotropic | sample_conditions_1 |
Software:
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution
AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking
TOPSPIN v3, Bruker Biospin - collection, processing
NMR spectrometers:
- BRUKER Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA37096 BAC03764 BAG59132 BAG60605 |
| EMBL | CAA09232 CAB09536 CAB64868 CAB66135 CAC14797 |
| GB | AAA62473 AAB87571 AAB94741 AAB94742 AAC48722 |
| REF | NP_000229 NP_001003145 NP_001032801 NP_001036187 NP_001092571 |
| SP | O08703 O08962 O35219 O54852 O54853 |
| TPG | DAA18406 DAA18407 DAA30311 DAA32713 |