BMRB Entry 17645
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17645
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Title: Solution structure of myosin VI lever arm extension PubMed: 22936804
Deposition date: 2011-05-13 Original release date: 2012-08-30
Authors: Feng, Wei; Yu, Cong; Zhang, Mingjie
Citation: Yu, Cong; Lou, Jizhong; Wu, Jingjing; Pan, Lifeng; Feng, Wei; Zhang, Mingjie. "Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes." J. Biol. Chem. 287, 35021-35035 (2012).
Assembly members:
myosin_VI_LAE, polymer, 88 residues, 9961.641 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
myosin_VI_LAE: QGPGSLVKVGTLKKRLDKFN
EVVSALKDGKPEVNRQIKNL
EISIDALMAKIKSTMMTREQ
IQKEYDALVKSSEDLLSALQ
KKKQQEEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 305 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 637 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | myosin VI lever arm extension | 1 |
Entities:
Entity 1, myosin VI lever arm extension 88 residues - 9961.641 Da.
Residues "QGPGS" are from the non-native tag
| 1 | GLN | GLY | PRO | GLY | SER | LEU | VAL | LYS | VAL | GLY | ||||
| 2 | THR | LEU | LYS | LYS | ARG | LEU | ASP | LYS | PHE | ASN | ||||
| 3 | GLU | VAL | VAL | SER | ALA | LEU | LYS | ASP | GLY | LYS | ||||
| 4 | PRO | GLU | VAL | ASN | ARG | GLN | ILE | LYS | ASN | LEU | ||||
| 5 | GLU | ILE | SER | ILE | ASP | ALA | LEU | MET | ALA | LYS | ||||
| 6 | ILE | LYS | SER | THR | MET | MET | THR | ARG | GLU | GLN | ||||
| 7 | ILE | GLN | LYS | GLU | TYR | ASP | ALA | LEU | VAL | LYS | ||||
| 8 | SER | SER | GLU | ASP | LEU | LEU | SER | ALA | LEU | GLN | ||||
| 9 | LYS | LYS | LYS | GLN | GLN | GLU | GLU | GLU |
Samples:
sample_1: myosin VI LAE 1.0 mM; D2O 100%; sodium phosphate 50 mM
sample_2: myosin VI LAE, [U-100% 15N], 1.0 mM; H2O 90%; D2O 10%
sample_3: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; H20 90%; D20 10%
sample_4: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; D2O 100%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_4 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
PIPP, Garrett - data analysis
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAD32219 |
| GB | AAB00194 AAI44918 AAI50674 EDL26433 EDL26434 |
| PRF | 2204386A |
| REF | NP_001034635 XP_001061392 XP_003508949 XP_004696356 XP_004696357 |
| SP | Q64331 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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