BMRB Entry 17492
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17492
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Title: Trn- peptide of the two-component bacteriocin Thuricin CD PubMed: 21526839
Deposition date: 2011-02-25 Original release date: 2011-05-05
Authors: Sit, Clarissa; McKay, Ryan; Hill, Colin; Ross, R.; Vederas, John
Citation: Sit, Clarissa; McKay, Ryan; Hill, Colin; Ross, R. Paul; Vederas, John. "The 3D Structure of Thuricin CD, a Two-Component Bacteriocin with Cysteine Sulfur to -Carbon Cross-links." J. Am. Chem. Soc. 133, 7680-7683 (2011).
Assembly members:
Thuricin, polymer, 30 residues, 2770.219 Da.
Natural source: Common Name: B.thuringiensis Taxonomy ID: 1428 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus thuringiensis
Experimental source: Production method: purified from the natural source Host organism: Bacillus thuringiensis
Entity Sequences (FASTA):
Thuricin: GNAACVIGCIGSCVISEGIG
SLVGTAFXLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 105 |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 177 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Thuricin | 1 |
Entities:
Entity 1, Thuricin 30 residues - 2770.219 Da.
| 1 | GLY | ASN | ALA | ALA | CYS | VAL | ILE | GLY | CYS | ILE | |
| 2 | GLY | SER | CYS | VAL | ILE | SER | GLU | GLY | ILE | GLY | |
| 3 | SER | LEU | VAL | GLY | THR | ALA | PHE | DTH | LEU | GLY |
Samples:
Trna: Trna, [U-99% 13C; U-99% 15N], 0.5 mM; DSS 100 uM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Trna | isotropic | sample_conditions_1 |
| 3D HNHA | Trna | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | Trna | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | Trna | isotropic | sample_conditions_1 |
| 3D HNCO | Trna | isotropic | sample_conditions_1 |
| rtm-HNCA | Trna | isotropic | sample_conditions_1 |
| 3D HNCACB | Trna | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | Trna | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | Trna | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
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