BMRB Entry 17485
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17485
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Title: Solution structure of Pf1 SID1-mSin3A PAH2 Complex PubMed: 21440557
Deposition date: 2011-02-23 Original release date: 2011-03-30
Authors: Senthil Kumar, Ganesan; Xie, Tao; Zhang, Yongbo; Radhakrishnan, Ishwar
Citation: Kumar, Ganesan Senthil; Xie, Tao; Zhang, Yongbo; Radhakrishnan, Ishwar. "Solution Structure of the mSin3A PAH2-Pf1 SID1 Complex: A Mad1/Mxd1-Like Interaction Disrupted by MRG15 in the Rpd3S/Sin3S Complex." J. Mol. Biol. 408, 987-1000 (2011).
Assembly members:
entity_1, polymer, 45 residues, 4993.651 Da.
entity_2, polymer, 94 residues, 10816.996 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: SNADYVQPQLRRPFELLIAA
AMERNPTQFQLPNELTCTTA
LPGSS
entity_2: SNASLQNNQPVEFNHAINYV
NKIKNRFQGQPDIYKAFLEI
LHTYQKEQRNAKEAGGNYTP
ALTEQEVYAQVARLFKNQED
LLSEFGQFLPDANS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 545 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 866 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 45 residues - 4993.651 Da.
| 1 | SER | ASN | ALA | ASP | TYR | VAL | GLN | PRO | GLN | LEU | ||||
| 2 | ARG | ARG | PRO | PHE | GLU | LEU | LEU | ILE | ALA | ALA | ||||
| 3 | ALA | MET | GLU | ARG | ASN | PRO | THR | GLN | PHE | GLN | ||||
| 4 | LEU | PRO | ASN | GLU | LEU | THR | CYS | THR | THR | ALA | ||||
| 5 | LEU | PRO | GLY | SER | SER |
Entity 2, entity_2 94 residues - 10816.996 Da.
| 1 | SER | ASN | ALA | SER | LEU | GLN | ASN | ASN | GLN | PRO | ||||
| 2 | VAL | GLU | PHE | ASN | HIS | ALA | ILE | ASN | TYR | VAL | ||||
| 3 | ASN | LYS | ILE | LYS | ASN | ARG | PHE | GLN | GLY | GLN | ||||
| 4 | PRO | ASP | ILE | TYR | LYS | ALA | PHE | LEU | GLU | ILE | ||||
| 5 | LEU | HIS | THR | TYR | GLN | LYS | GLU | GLN | ARG | ASN | ||||
| 6 | ALA | LYS | GLU | ALA | GLY | GLY | ASN | TYR | THR | PRO | ||||
| 7 | ALA | LEU | THR | GLU | GLN | GLU | VAL | TYR | ALA | GLN | ||||
| 8 | VAL | ALA | ARG | LEU | PHE | LYS | ASN | GLN | GLU | ASP | ||||
| 9 | LEU | LEU | SER | GLU | PHE | GLY | GLN | PHE | LEU | PRO | ||||
| 10 | ASP | ALA | ASN | SER |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.75 – 1.7 mM; entity_2, [U-100% 13C; U-100% 15N], 0.75 – 1.7 mM; H2O, [U-100% 13C; U-100% 15N], 90%; D2O, [U-100% 13C; U-100% 15N], 10%
sample_conditions_1: ionic strength: 0 M; pH: 6.00; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v1.2, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O, . - refinement, structure solution
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 18000 4635 |
| PDB | |
| DBJ | BAD18713 BAE00370 BAE24837 BAE33212 BAE41602 |
| GB | AAH01657 AAH43080 AAI10883 AAI21044 AAI21045 AAK95854 |
| REF | NP_001013135 NP_001028733 NP_001179060 NP_001253214 NP_001277060 XP_004660420 XP_005994973 XP_007239639 XP_009197516 |
| SP | Q5SPL2 Q96QT6 |
| TPG | DAA18992 |
Download simulated HSQC data in one of the following formats:
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