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BMRB Entry 17354

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17354
MolProbity Validation Chart

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Title: high calcium androcam   PubMed: 22706934

Deposition date: 2010-12-08 Original release date: 2012-07-25

Authors: Joshi, Mehul; Moran, Sean; MacKenzie, Kevin

Citation: Joshi, Mehul; Moran, Sean; MacKenzie, Kevin. "H, 15N and 13C chemical shifts of the D. melanogaster myosin VI light chain androcam in high calcium."  Biomol. NMR Assignments 7, 171-174 (2013).

Assembly members:
androcam, polymer, 148 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
androcam: MSELTEEQIAEFKDAFVQFD KEGTGKIATRELGTLMRTLG QNPTEAELQDLIAEAENNNN GQLNFTEFCGIMAKQMRETD TEEEMREAFKIFDRDGDGFI SPAELRFVMINLGEKVTDEE IDEMIREADFDGDGMINYEE FVWMISQK

Data sets:
Data typeCount
13C chemical shifts587
15N chemical shifts162
1H chemical shifts1038

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1androcam1
2calcium ions2

Entities:

Entity 1, androcam 148 residues - Formula weight is not available

1   METSERGLULEUTHRGLUGLUGLNILEALA
2   GLUPHELYSASPALAPHEVALGLNPHEASP
3   LYSGLUGLYTHRGLYLYSILEALATHRARG
4   GLULEUGLYTHRLEUMETARGTHRLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   LEUILEALAGLUALAGLUASNASNASNASN
7   GLYGLNLEUASNPHETHRGLUPHECYSGLY
8   ILEMETALALYSGLNMETARGGLUTHRASP
9   THRGLUGLUGLUMETARGGLUALAPHELYS
10   ILEPHEASPARGASPGLYASPGLYPHEILE
11   SERPROALAGLULEUARGPHEVALMETILE
12   ASNLEUGLYGLULYSVALTHRASPGLUGLU
13   ILEASPGLUMETILEARGGLUALAASPPHE
14   ASPGLYASPGLYMETILEASNTYRGLUGLU
15   PHEVALTRPMETILESERGLNLYS

Entity 2, calcium ions - Ca - 40.078 Da.

1   CA

Samples:

sample_1: androcam, [U-100% 13C; U-100% 15N], 1.8 mM; Calcium chloride 10 mM; D2O 10%; H2O 90%; potassium chloride 10 mM; TRIS 10 mM; DTT 10 mM; TSP 0.1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17353
PDB
EMBL CAA53630
GB AAF56511 AAL48405 ACL87451 ACL91891 ACZ95031
PRF 2021248D
REF NP_001163737 NP_476988 XP_001981747 XP_002041300 XP_002098987
SP P49258

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts