BMRB Entry 16365
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16365
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution NMR structure of yeast protein YOR252W [residues 38-178]: Northeast Structural Genomics Consortium target YT654
Deposition date: 2009-06-26 Original release date: 2012-08-03
Authors: Cort, John; Yee, Adelinda; Liu, Chengsong; Arrowsmith, Cheryl; Kennedy, Michael
Citation: Cort, John; Yee, Adelinda; Liu, Chengsong; Arrowsmith, Cheryl; Kennedy, Michael. "Solution NMR structure of yeast protein YOR252W" Not known ., .-..
Assembly members:
YOR252W, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
YOR252W: MREDKIAAKKKLHQDKRVHE
LARVKFMQDVVNSDTFKGQP
IFDHAHTREFIQSFIERDDT
ELDELKKKRRSNRPPSNRQV
LLQQRRDQELKEFKAGFLCP
DLSDAKNMEFLRNWNGTFGL
LNTLRLIRINDKGEQVVGGN
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 514 |
| 15N chemical shifts | 133 |
| 1H chemical shifts | 775 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YOR252W | 1 |
Entities:
Entity 1, YOR252W 141 residues - Formula weight is not available
An N-term His purification tag was cleaved.
| 1 | MET | ARG | GLU | ASP | LYS | ILE | ALA | ALA | LYS | LYS | ||||
| 2 | LYS | LEU | HIS | GLN | ASP | LYS | ARG | VAL | HIS | GLU | ||||
| 3 | LEU | ALA | ARG | VAL | LYS | PHE | MET | GLN | ASP | VAL | ||||
| 4 | VAL | ASN | SER | ASP | THR | PHE | LYS | GLY | GLN | PRO | ||||
| 5 | ILE | PHE | ASP | HIS | ALA | HIS | THR | ARG | GLU | PHE | ||||
| 6 | ILE | GLN | SER | PHE | ILE | GLU | ARG | ASP | ASP | THR | ||||
| 7 | GLU | LEU | ASP | GLU | LEU | LYS | LYS | LYS | ARG | ARG | ||||
| 8 | SER | ASN | ARG | PRO | PRO | SER | ASN | ARG | GLN | VAL | ||||
| 9 | LEU | LEU | GLN | GLN | ARG | ARG | ASP | GLN | GLU | LEU | ||||
| 10 | LYS | GLU | PHE | LYS | ALA | GLY | PHE | LEU | CYS | PRO | ||||
| 11 | ASP | LEU | SER | ASP | ALA | LYS | ASN | MET | GLU | PHE | ||||
| 12 | LEU | ARG | ASN | TRP | ASN | GLY | THR | PHE | GLY | LEU | ||||
| 13 | LEU | ASN | THR | LEU | ARG | LEU | ILE | ARG | ILE | ASN | ||||
| 14 | ASP | LYS | GLY | GLU | GLN | VAL | VAL | GLY | GLY | ASN | ||||
| 15 | GLU |
Samples:
sample_1: YOR252W, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 300 mM; DTT 10 mM; benzamidine 1 mM; sodium azide 0.01%; H2O 93%; D2O 7%
sample_2: YOR252W, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 300 mM; DTT 10 mM; benzamidine 1 mM; sodium azide 0.01%; D2O 100%
sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 4D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
AutoStruct, Huang, Tejero, Powers and Montelione - refinement, structure solution
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, refinement
PSVS, Bhattacharya and Montelione - refinement
SPARKY, Goddard - chemical shift assignment, peak picking
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA26563 |
| EMBL | CAA99474 CAY86533 |
| GB | AHY77532 AJP41762 AJT71184 AJT71672 AJT72162 |
| REF | NP_014895 |
| SP | Q08687 |
| TPG | DAA11019 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts