BMRB Entry 16085
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16085
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Title: 1H, 13C, 15N Chemical Shift Assignment for Saccharomyces cerevisiae Acyl Carrier Protein. PubMed: 19636964
Deposition date: 2008-12-23 Original release date: 2009-04-16
Authors: Perez, Daniel
Citation: Perez, Daniel; Wider, Gerhard. "1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase" Biomol. NMR Assignments 3, 133-136 (2009).
Assembly members:
Acyl_Carrier_Protein, polymer, 183 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Acyl_Carrier_Protein: MGSSHHHHHHENLYFQSNAE
IADEPVKASLLLHVLVAHKL
KKSLDSIPMSKTIKDLVGGK
STVQNEILGDLGKEFGTTPE
KPEETPLEELAETFQDTFSG
ALGKQSSSLLSRLISSKMPG
GFTITVARKYLQTRWGLPSG
RQDGVLLVALSNEPAARLGS
EADAKAFLDSMAQKYASIVG
VDL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 725 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 1181 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ACP single polypeptide chain | 1 |
Entities:
Entity 1, ACP single polypeptide chain 183 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLU | ASN | LEU | TYR | PHE | GLN | SER | ASN | ALA | GLU | ||||
| 3 | ILE | ALA | ASP | GLU | PRO | VAL | LYS | ALA | SER | LEU | ||||
| 4 | LEU | LEU | HIS | VAL | LEU | VAL | ALA | HIS | LYS | LEU | ||||
| 5 | LYS | LYS | SER | LEU | ASP | SER | ILE | PRO | MET | SER | ||||
| 6 | LYS | THR | ILE | LYS | ASP | LEU | VAL | GLY | GLY | LYS | ||||
| 7 | SER | THR | VAL | GLN | ASN | GLU | ILE | LEU | GLY | ASP | ||||
| 8 | LEU | GLY | LYS | GLU | PHE | GLY | THR | THR | PRO | GLU | ||||
| 9 | LYS | PRO | GLU | GLU | THR | PRO | LEU | GLU | GLU | LEU | ||||
| 10 | ALA | GLU | THR | PHE | GLN | ASP | THR | PHE | SER | GLY | ||||
| 11 | ALA | LEU | GLY | LYS | GLN | SER | SER | SER | LEU | LEU | ||||
| 12 | SER | ARG | LEU | ILE | SER | SER | LYS | MET | PRO | GLY | ||||
| 13 | GLY | PHE | THR | ILE | THR | VAL | ALA | ARG | LYS | TYR | ||||
| 14 | LEU | GLN | THR | ARG | TRP | GLY | LEU | PRO | SER | GLY | ||||
| 15 | ARG | GLN | ASP | GLY | VAL | LEU | LEU | VAL | ALA | LEU | ||||
| 16 | SER | ASN | GLU | PRO | ALA | ALA | ARG | LEU | GLY | SER | ||||
| 17 | GLU | ALA | ASP | ALA | LYS | ALA | PHE | LEU | ASP | SER | ||||
| 18 | MET | ALA | GLN | LYS | TYR | ALA | SER | ILE | VAL | GLY | ||||
| 19 | VAL | ASP | LEU |
Samples:
sample_1: Acyl Carrier Protein, [U-13C; U-15N], 1 mM; KH2PO4 : K2HPO4 (0.685 : 0.315) buffer 52 mM
sample_conditions_1: ionic strength: 0.052 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - data analysis
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| DBJ | GAA26747 |
| EMBL | CAA54218 CAA64256 CAA97948 CAY86729 |
| GB | AAA34601 AHY77952 AJP41920 AJU23364 AJU24052 |
| REF | NP_015093 |
| SP | P19097 |
| TPG | DAA11205 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts