BMRB Entry 16056
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16056
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Title: STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM XI OF THE NHE1 ISOFORM OF THE NA+/H+ EXCHANGER PubMed: 19176522
Deposition date: 2008-12-09 Original release date: 2009-01-21
Authors: Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry
Citation: Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of TM XI of the NHE1 Isoform of the Na+/H+ Exchanger" J. Biol. Chem. 284, 11546-11556 (2009).
Assembly members:
TMXI, polymer, 28 residues, 2860.407 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TMXI: XKDQFIIAYGGLRGAIAFSL
GYLLDKKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 208 |
| 13C chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TMXI | 1 |
Entities:
Entity 1, TMXI 28 residues - 2860.407 Da.
N terminal acetate cap, C terminal NH2 cap
| 1 | ACE | LYS | ASP | GLN | PHE | ILE | ILE | ALA | TYR | GLY | ||||
| 2 | GLY | LEU | ARG | GLY | ALA | ILE | ALA | PHE | SER | LEU | ||||
| 3 | GLY | TYR | LEU | LEU | ASP | LYS | LYS | NH2 |
Samples:
sample_1: DPC 150 mM; DSS 0.25 mM; TMXI 2 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 303 K; pH: 5; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution, refinement
VNMRJ, Varian - collection
NMRView, Johnson, One Moon Scientific - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz