BMRB Entry 16015
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16015
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Title: Structure of protein complex PubMed: 19214187
Deposition date: 2008-10-30 Original release date: 2009-02-16
Authors: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter
Citation: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter. "Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation" EMBO J. 28, 948-958 (2009).
Assembly members:
TAZ2, polymer, 92 residues, Formula weight is not available
STAT1, polymer, 45 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TAZ2: SPQESRRLSIQRCIQSLVHA
CQCRNANCSLPSCQKMKRVV
QHTKGCKRKTNGGCPVCKQL
IALCCYHAKHCQENKCPVPF
CLNIKHKLRQQQ
STAT1: GSHMSEVHPSRLQTTDNLLP
MSPEEFDEVSRIVGSVEFDS
MMNTV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 434 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 933 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAZ2 | 1 |
| 2 | STAT1 | 2 |
| 3 | ZINC ION_1 | 3 |
| 4 | ZINC ION_2 | 3 |
| 5 | ZINC ION_3 | 3 |
Entities:
Entity 1, TAZ2 92 residues - Formula weight is not available
| 1 | SER | PRO | GLN | GLU | SER | ARG | ARG | LEU | SER | ILE | ||||
| 2 | GLN | ARG | CYS | ILE | GLN | SER | LEU | VAL | HIS | ALA | ||||
| 3 | CYS | GLN | CYS | ARG | ASN | ALA | ASN | CYS | SER | LEU | ||||
| 4 | PRO | SER | CYS | GLN | LYS | MET | LYS | ARG | VAL | VAL | ||||
| 5 | GLN | HIS | THR | LYS | GLY | CYS | LYS | ARG | LYS | THR | ||||
| 6 | ASN | GLY | GLY | CYS | PRO | VAL | CYS | LYS | GLN | LEU | ||||
| 7 | ILE | ALA | LEU | CYS | CYS | TYR | HIS | ALA | LYS | HIS | ||||
| 8 | CYS | GLN | GLU | ASN | LYS | CYS | PRO | VAL | PRO | PHE | ||||
| 9 | CYS | LEU | ASN | ILE | LYS | HIS | LYS | LEU | ARG | GLN | ||||
| 10 | GLN | GLN |
Entity 2, STAT1 45 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | SER | GLU | VAL | HIS | PRO | SER | ||||
| 2 | ARG | LEU | GLN | THR | THR | ASP | ASN | LEU | LEU | PRO | ||||
| 3 | MET | SER | PRO | GLU | GLU | PHE | ASP | GLU | VAL | SER | ||||
| 4 | ARG | ILE | VAL | GLY | SER | VAL | GLU | PHE | ASP | SER | ||||
| 5 | MET | MET | ASN | THR | VAL |
Entity 3, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: TAZ2, [U-100% 15N], 1 mM; STAT1; ZINC_ION; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 900 MHz
Related Database Links:
| BMRB | 16318 |
| PDB | |
| DBJ | BAE06125 BAI45616 |
| EMBL | CAF96470 CAG06082 CDQ92618 |
| GB | AAB28651 AAC17736 AAC51331 AAC51340 AAC51770 |
| PRF | 1923401A |
| REF | NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001192159 |
| SP | P45481 Q6JHU9 Q92793 |
| TPG | DAA15549 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts