BMRB Entry 16002
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16002
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Title: cytoskeletal complex PubMed: 19074766
Deposition date: 2008-10-24 Original release date: 2009-04-04
Authors: Ithychanda, Sujay Subbayya
Citation: Ithychanda, Sujay Subbayya; Das, Mitali; Ma, Yan-Qing; Ding, Keyang; Wang, Xiaoxia; Gupta, Sudhiranjan; Wu, Chuanyue; Plow, Edward; Qin, Jun. "Migfilin, a molecular switch in regulation of integrin activation" J. Biol. Chem. 284, 4713-4722 (2009).
Assembly members:
entity_1, polymer, 119 residues, 12434.841 Da.
entity_2, polymer, 24 residues, 2661.174 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GIPEFFQFTVGPLGEGGAHK
VRAGGTGLERGVAGVPAEFS
IWTREAGAGGLSIAVEGPSK
AEIAFEDRKDGSCGVSYVVQ
EPGDYEVSIKFNDEHIPDSP
FVVPVASLSDDARRLTVTS
entity_2: MASKPEKRVASSVFITLAPP
RRDV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 748 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 119 residues - 12434.841 Da.
| 1 | GLY | ILE | PRO | GLU | PHE | PHE | GLN | PHE | THR | VAL | ||||
| 2 | GLY | PRO | LEU | GLY | GLU | GLY | GLY | ALA | HIS | LYS | ||||
| 3 | VAL | ARG | ALA | GLY | GLY | THR | GLY | LEU | GLU | ARG | ||||
| 4 | GLY | VAL | ALA | GLY | VAL | PRO | ALA | GLU | PHE | SER | ||||
| 5 | ILE | TRP | THR | ARG | GLU | ALA | GLY | ALA | GLY | GLY | ||||
| 6 | LEU | SER | ILE | ALA | VAL | GLU | GLY | PRO | SER | LYS | ||||
| 7 | ALA | GLU | ILE | ALA | PHE | GLU | ASP | ARG | LYS | ASP | ||||
| 8 | GLY | SER | CYS | GLY | VAL | SER | TYR | VAL | VAL | GLN | ||||
| 9 | GLU | PRO | GLY | ASP | TYR | GLU | VAL | SER | ILE | LYS | ||||
| 10 | PHE | ASN | ASP | GLU | HIS | ILE | PRO | ASP | SER | PRO | ||||
| 11 | PHE | VAL | VAL | PRO | VAL | ALA | SER | LEU | SER | ASP | ||||
| 12 | ASP | ALA | ARG | ARG | LEU | THR | VAL | THR | SER |
Entity 2, entity_2 24 residues - 2661.174 Da.
| 1 | MET | ALA | SER | LYS | PRO | GLU | LYS | ARG | VAL | ALA | ||||
| 2 | SER | SER | VAL | PHE | ILE | THR | LEU | ALA | PRO | PRO | ||||
| 3 | ARG | ARG | ASP | VAL |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.1-1 mM; entity_2 0.1-1 mM
sample_conditions_1: ionic strength: 0.025 M; pH: 6.4; pressure: 1 atm; temperature: 302 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB55115 BAE32761 BAE41598 BAE41798 BAG51492 |
| EMBL | CAA47354 |
| GB | AAH04777 AAH19895 AAO15549 AAO49012 AAQ08090 |
| PIR | A38203 |
| REF | NP_001019386 NP_001019387 NP_001156728 NP_060026 NP_598515 |
| SP | Q71FD7 Q8WUP2 |
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