BMRB Entry 15700
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15700
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Title: solution struture of human N-terminal domain of pirh2/Northeast Structure Genomics Consortium (NESG) target HT2A PubMed: 19043414
Deposition date: 2008-03-31 Original release date: 2009-04-03
Authors: Wu, Bin; Lemak, Alexander; Sheng, Yi; Karra, Murthy; Srisailam, Sampath; Sunnerhagen, Maria; Arrowsmith, Cherl
Citation: Sheng, Yi; Laister, Rob; Lemak, Alexander; Wu, Bin; Tai, Elizabeth; Duan, Shili; Lukin, Jonathan; Sunnerhagen, Maria; Srisailam, Sampath; Karra, Murthy; Benchimol, Sam; Arrowsmith, Cheryl. "Molecular basis of Pirh2-mediated p53 ubiquitylation" Nat. Struct. Mol. Biol. 15, 1334-1342 (2008).
Assembly members:
human_N-termal_doamin_of_pirh2, polymer, 137 residues, 15905.286 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human_N-termal_doamin_of_pirh2: MAATAREDGATGEERGQRGC
EHYDRGCLLKAPCCDKLYTC
RLCHDNNEDHQLDRFKVKEV
QCINCEKIQHAQQTCEECST
LFGEYYCDICHLFDKDKKQY
HCENCGICRIGPKEDFFHCL
KCNLCLAMNLQGRHKCI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 519 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 833 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
| 4 | ZINC ION_3 | 2 |
| 5 | ZINC ION_4 | 2 |
| 6 | ZINC ION_5 | 2 |
| 7 | ZINC ION_6 | 2 |
Entities:
Entity 1, entity_1 137 residues - 15905.286 Da.
| 1 | MET | ALA | ALA | THR | ALA | ARG | GLU | ASP | GLY | ALA | ||||
| 2 | THR | GLY | GLU | GLU | ARG | GLY | GLN | ARG | GLY | CYS | ||||
| 3 | GLU | HIS | TYR | ASP | ARG | GLY | CYS | LEU | LEU | LYS | ||||
| 4 | ALA | PRO | CYS | CYS | ASP | LYS | LEU | TYR | THR | CYS | ||||
| 5 | ARG | LEU | CYS | HIS | ASP | ASN | ASN | GLU | ASP | HIS | ||||
| 6 | GLN | LEU | ASP | ARG | PHE | LYS | VAL | LYS | GLU | VAL | ||||
| 7 | GLN | CYS | ILE | ASN | CYS | GLU | LYS | ILE | GLN | HIS | ||||
| 8 | ALA | GLN | GLN | THR | CYS | GLU | GLU | CYS | SER | THR | ||||
| 9 | LEU | PHE | GLY | GLU | TYR | TYR | CYS | ASP | ILE | CYS | ||||
| 10 | HIS | LEU | PHE | ASP | LYS | ASP | LYS | LYS | GLN | TYR | ||||
| 11 | HIS | CYS | GLU | ASN | CYS | GLY | ILE | CYS | ARG | ILE | ||||
| 12 | GLY | PRO | LYS | GLU | ASP | PHE | PHE | HIS | CYS | LEU | ||||
| 13 | LYS | CYS | ASN | LEU | CYS | LEU | ALA | MET | ASN | LEU | ||||
| 14 | GLN | GLY | ARG | HIS | LYS | CYS | ILE |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_2: human N-termal doamin of pirh2, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 150 mM; Zncl2 10 uM; DTT 0.8 mM
sample_1: human N-termal doamin of pirh2, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 150 mM; Zncl2 10 uM; DTT 0.8 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D aromatic 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.95, Goddard - data analysis
ABACUS, Alexander Lemak, Cherl H. Arrowsmith - chemical shift assignment, structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoStruct v2.1.0, Huang, Tejero, Powers and Montelione - Validation
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92309 BAG52375 BAI46763 |
| EMBL | CAB43290 |
| GB | AAH47393 AAK96896 AAL09356 AAL76101 AAX78233 |
| REF | NP_001009922 NP_001248474 NP_001265467 NP_056251 XP_001154449 |
| SP | Q96PM5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts