BMRB Entry 15674
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15674
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Title: Solution NMR structure of the chromobox protein homolog 7
Deposition date: 2008-02-25 Original release date: 2008-03-05
Authors: Kaustov, Lilia; Lemak, Alexander; Quyang, Hui; Gutmanas, Alexandras; Fares, Christophe; Bountra, Chas; Weigelt, Johan; Ravichandran, Mani; Loppnau, Peter; Edwards, Aled; Min, Jinrong; Arrowsmith, Cheryl
Citation: Kaustov, Lilia; Lemak, Alexander; Quyang, Hui; Gutmanas, Alexandras; Fares, Christophe; Bountra, Chas; Weigelt, Johan; Ravichandran, Mani; Loppnau, Peter; Edwards, Aled; Min, Jinrong; Arrowsmith, Cheryl. "Solution NMR structure of the chromobox protein homolog 7" . ., .-..
Assembly members:
cbx7, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cbx7: MHHHHHHSSGRENLYFQGEQ
VFAVESIRKKRVRKGKVEYL
VKWKGWPPKYSTWEPEEHIL
DPRLVMAYEEKEE
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 248 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 410 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chromodomain | 1 |
Entities:
Entity 1, chromodomain 73 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | GLY | ||||
| 2 | ARG | GLU | ASN | LEU | TYR | PHE | GLN | GLY | GLU | GLN | ||||
| 3 | VAL | PHE | ALA | VAL | GLU | SER | ILE | ARG | LYS | LYS | ||||
| 4 | ARG | VAL | ARG | LYS | GLY | LYS | VAL | GLU | TYR | LEU | ||||
| 5 | VAL | LYS | TRP | LYS | GLY | TRP | PRO | PRO | LYS | TYR | ||||
| 6 | SER | THR | TRP | GLU | PRO | GLU | GLU | HIS | ILE | LEU | ||||
| 7 | ASP | PRO | ARG | LEU | VAL | MET | ALA | TYR | GLU | GLU | ||||
| 8 | LYS | GLU | GLU |
Samples:
sample_1: phosphate 10 mM; sodium chloride 300 mM; DTT 2 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D aromat 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 500 MHz
Related Database Links:
| BMRB | 16778 17072 17079 |
| PDB | |
| DBJ | BAI46775 |
| GB | AAH51773 AAX94038 AAY68391 ABZ92314 AIC56058 |
| REF | NP_001179547 NP_001247702 NP_783640 XP_002743799 XP_003126060 |
| SP | O95931 |
| TPG | DAA29144 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts