BMRB Entry 15665
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15665
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Title: CONFIRMATIONAL ANALYSIS OF THE BROAD-SPECTRUM ANTIBACTERIAL PEPTIDE, RANTUERIN-2CSA: IDENTIFICATION OF A FULL LENGTH HELIX-TURN-HELIX MOTIF PubMed: 18387372
Deposition date: 2008-02-19 Original release date: 2008-06-27
Authors: Hewage, Chandralal; Subasinghage, Anusha; Conlon, Michael
Citation: Subasinghage, Anusha; Conlon, J.; Hewage, Chandralal. "Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: Identification of a full length helix-turn-helix motif." Biochim. Biophys. Acta 1784, 924-929 (2008).
Assembly members:
ranatuerin-2CSa, polymer, 32 residues, 3256.000 Da.
Natural source: Common Name: Rana cascadae Taxonomy ID: 160497 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana cascadae
Experimental source: Production method: chemical synthesis Host organism: Rana cascadae
Entity Sequences (FASTA):
ranatuerin-2CSa: GILSSFKGVAKGVAKDLAGK
LLETLKCKITGC
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 215 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ranatuerin-2CSa | 1 |
Entities:
Entity 1, ranatuerin-2CSa 32 residues - 3256.000 Da.
| 1 | GLY | ILE | LEU | SER | SER | PHE | LYS | GLY | VAL | ALA | ||||
| 2 | LYS | GLY | VAL | ALA | LYS | ASP | LEU | ALA | GLY | LYS | ||||
| 3 | LEU | LEU | GLU | THR | LEU | LYS | CYS | LYS | ILE | THR | ||||
| 4 | GLY | CYS |
Samples:
Ranatuerin-2CSa: Peptide 1.5-2.0 mM; TFE-d3/H2O 1:1 v/v
Ranatuerin-2CSa_c: pH: 3.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | Ranatuerin-2CSa | isotropic | Ranatuerin-2CSa_c |
| 2D 1H-1H NOESY | Ranatuerin-2CSa | isotropic | Ranatuerin-2CSa_c |
| 2D DQF-COSY | Ranatuerin-2CSa | isotropic | Ranatuerin-2CSa_c |
Software:
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - processing
SYBYL v6.8.1, Tripos - refinement
xwinnmr v3.5, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB |