BMRB Entry 15559
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15559
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Title: NS4A(40-69) PubMed: 19357161
Deposition date: 2007-11-19 Original release date: 2009-05-14
Authors: Montserret, Roland; Penin, Francois
Citation: Gouttenoire, Jerome; Castet, Valerie; Montserret, Roland; Arora, Naveen; Raussens, Vincent; Ruysschaert, Jean-Marie; Diesis, Eric; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B" J. Virol. 83, 6257-6268 (2009).
Assembly members:
NS4B(40-69), polymer, 30 residues, 3601.153 Da.
Natural source: Common Name: not available Taxonomy ID: 11103 Superkingdom: not available Kingdom: Viruses Genus/species: not available Hepacivirus
Experimental source: Production method: chemical synthesis Host organism: none
Entity Sequences (FASTA):
NS4B(40-69): QTNWQKLEVFWAKHMWNFIS
GIQYLAGLST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 106 |
| 1H chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 30 residues - 3601.153 Da.
| 1 | GLN | THR | ASN | TRP | GLN | LYS | LEU | GLU | VAL | PHE | |
| 2 | TRP | ALA | LYS | HIS | MET | TRP | ASN | PHE | ILE | SER | |
| 3 | GLY | ILE | GLN | TYR | LEU | ALA | GLY | LEU | SER | THR |
Samples:
sample_1: NS4B(40-69) 1 mM; Trifluoro Ethanol D2OH 50 % v/v; H2O 50 % v/v
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection
SPARKY, Goddard - chemical shift assignment
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Varian UnityPlus 500 MHz