BMRB Entry 15449
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15449
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Title: Type I rat fatty acid synthase acyl carrier protein (ACP) domain PubMed: 17971456
Deposition date: 2007-08-30 Original release date: 2008-01-28
Authors: Ploskon, E.; Arthur, C.; Evans, S.; Williams, C.; Crosby, J.; Crump, M.
Citation: Ploskon, E.; Arthur, C.; Evans, S.; Williams, C.; Crosby, J.; Simpson, T.; Crump, M.. "A Mammalian Type I fatty acid synthase acyl carrier protein domain does not bind acyl chains" J. Biol. Chem. 283, 518-528 (2008).
Assembly members:
Fatty_acid_synthase_(EC_2.3.1.85), polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Fatty_acid_synthase_(EC_2.3.1.85): GDGEAQRDLVKAVAHILGIR
DLAGINLDSSLADLGLDSLM
GVEVRQILEREHDLVLPIRE
VRQLTLRKLQEMSSKAGSDT
ELAAPKSKN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 283 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 609 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 89 residues - Formula weight is not available
| 1 | GLY | ASP | GLY | GLU | ALA | GLN | ARG | ASP | LEU | VAL | ||||
| 2 | LYS | ALA | VAL | ALA | HIS | ILE | LEU | GLY | ILE | ARG | ||||
| 3 | ASP | LEU | ALA | GLY | ILE | ASN | LEU | ASP | SER | SER | ||||
| 4 | LEU | ALA | ASP | LEU | GLY | LEU | ASP | SER | LEU | MET | ||||
| 5 | GLY | VAL | GLU | VAL | ARG | GLN | ILE | LEU | GLU | ARG | ||||
| 6 | GLU | HIS | ASP | LEU | VAL | LEU | PRO | ILE | ARG | GLU | ||||
| 7 | VAL | ARG | GLN | LEU | THR | LEU | ARG | LYS | LEU | GLN | ||||
| 8 | GLU | MET | SER | SER | LYS | ALA | GLY | SER | ASP | THR | ||||
| 9 | GLU | LEU | ALA | ALA | PRO | LYS | SER | LYS | ASN |
Samples:
sample: ACP1 – 2 mM; sodium deuteroacetate 20 mM; sodium azide 1 mM; DTT 1 mM; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
ANALYSIS, CCPN - data analysis
ARIA v1.2, Linge, O, . - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA31780 CAA31882 CAA44679 CAA44680 |
| GB | AAA41144 AAA41145 AAA57219 EDM06908 EDM06909 |
| REF | NP_059028 |
| SP | P12785 |
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