BMRB Entry 15431
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15431
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Title: NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia PubMed: 19636863
Deposition date: 2007-08-15 Original release date: 2007-11-16
Authors: Ulmer, Tobias
Citation: Hung, Kuo-Chan; Mac, Thien-Thi; Ulmer, Tobias. "NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia" Biomol. NMR Assignments 1, 195-196 (2007).
Assembly members:
DsbH, polymer, 144 residues, 16487.906 Da.
Natural source: Common Name: Chlamydia pneumoniae Taxonomy ID: 83558 Superkingdom: Bacteria Kingdom: not available Genus/species: Chlamydia pneumoniae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DsbH: ARRRASGENLQQTRPIAAAN
LQWESYAEALEHSKQDHKPI
GLFFTGSDWCMWCIKMQDQI
LQSSEFKHFAGVHLHMVEVD
FPQKNHQPEEQRQKNQELKA
QYKVTGFPELVFIDAEGKQL
ARMGFEPGGGAAYVSKVKSA
LKLR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 582 |
| 15N chemical shifts | 141 |
| 1H chemical shifts | 880 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polypeptide chain | 1 |
Entities:
Entity 1, polypeptide chain 144 residues - 16487.906 Da.
| 1 | ALA | ARG | ARG | ARG | ALA | SER | GLY | GLU | ASN | LEU | ||||
| 2 | GLN | GLN | THR | ARG | PRO | ILE | ALA | ALA | ALA | ASN | ||||
| 3 | LEU | GLN | TRP | GLU | SER | TYR | ALA | GLU | ALA | LEU | ||||
| 4 | GLU | HIS | SER | LYS | GLN | ASP | HIS | LYS | PRO | ILE | ||||
| 5 | GLY | LEU | PHE | PHE | THR | GLY | SER | ASP | TRP | CYS | ||||
| 6 | MET | TRP | CYS | ILE | LYS | MET | GLN | ASP | GLN | ILE | ||||
| 7 | LEU | GLN | SER | SER | GLU | PHE | LYS | HIS | PHE | ALA | ||||
| 8 | GLY | VAL | HIS | LEU | HIS | MET | VAL | GLU | VAL | ASP | ||||
| 9 | PHE | PRO | GLN | LYS | ASN | HIS | GLN | PRO | GLU | GLU | ||||
| 10 | GLN | ARG | GLN | LYS | ASN | GLN | GLU | LEU | LYS | ALA | ||||
| 11 | GLN | TYR | LYS | VAL | THR | GLY | PHE | PRO | GLU | LEU | ||||
| 12 | VAL | PHE | ILE | ASP | ALA | GLU | GLY | LYS | GLN | LEU | ||||
| 13 | ALA | ARG | MET | GLY | PHE | GLU | PRO | GLY | GLY | GLY | ||||
| 14 | ALA | ALA | TYR | VAL | SER | LYS | VAL | LYS | SER | ALA | ||||
| 15 | LEU | LYS | LEU | ARG |
Samples:
sample_1: DsbH, [U-99% 13C; U-99% 15N], 1.0 mM; NaH2PO4/Na2HPO4 50 mM; NaN3 0.02 % w/v; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 298.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | anisotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA99134 |
| EMBL | CRI33456 CRI36320 CRI37446 CRI38577 CRI39708 |
| GB | AAD19064 AAF38723 AAP98887 ACZ32813 ETR79697 |
| REF | NP_225121 WP_010883561 |
| SP | Q9Z6Y0 |
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