BMRB Entry 15037
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15037
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Title: Solution structure of human beta-microseminoprotein PubMed: 16930619
Deposition date: 2006-11-17 Original release date: 2007-01-10
Authors: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbj rn
Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structure of human and porcine beta-microseminoprotein." J. Mol. Biol. 362, 502-515 (2006).
Assembly members:
hMSP, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
hMSP: SCYFIPNEGVPGDSTRKCMD
LKGNKHPINSEWQTDNCETC
TCYETEISCCILVSTPVGYD
KDNCQRIFKKEDCKYIVVEK
KDPKKTCSVSEWII
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 467 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 94 residues - Formula weight is not available
| 1 | SER | CYS | TYR | PHE | ILE | PRO | ASN | GLU | GLY | VAL | ||||
| 2 | PRO | GLY | ASP | SER | THR | ARG | LYS | CYS | MET | ASP | ||||
| 3 | LEU | LYS | GLY | ASN | LYS | HIS | PRO | ILE | ASN | SER | ||||
| 4 | GLU | TRP | GLN | THR | ASP | ASN | CYS | GLU | THR | CYS | ||||
| 5 | THR | CYS | TYR | GLU | THR | GLU | ILE | SER | CYS | CYS | ||||
| 6 | ILE | LEU | VAL | SER | THR | PRO | VAL | GLY | TYR | ASP | ||||
| 7 | LYS | ASP | ASN | CYS | GLN | ARG | ILE | PHE | LYS | LYS | ||||
| 8 | GLU | ASP | CYS | LYS | TYR | ILE | VAL | VAL | GLU | LYS | ||||
| 9 | LYS | ASP | PRO | LYS | LYS | THR | CYS | SER | VAL | SER | ||||
| 10 | GLU | TRP | ILE | ILE |
Samples:
sample_1: beta-microseminoprotein3.4 – 4.2 mM
sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- VARIAN INOVA 600 MHz