BMRB Entry 11378
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11378
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Title: Solution structure of the third zinc finger domain of Zinc finger protein 278
Deposition date: 2010-09-08 Original release date: 2011-09-07
Authors: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the third zinc finger domain of Zinc finger protein 278" . ., .-..
Assembly members:
zinc finger domain, polymer, 45 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
zinc finger domain: GSSGSSGEKPYSCPVCGLRF
KRKDRMSYHVRSHDGSVGKS
GPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 159 |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 231 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zinc finger domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, zinc finger domain 45 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | LYS | PRO | ||||
| 2 | TYR | SER | CYS | PRO | VAL | CYS | GLY | LEU | ARG | PHE | ||||
| 3 | LYS | ARG | LYS | ASP | ARG | MET | SER | TYR | HIS | VAL | ||||
| 4 | ARG | SER | HIS | ASP | GLY | SER | VAL | GLY | LYS | SER | ||||
| 5 | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zinc finger domain, [U-13C; U-15N], 0.97 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20060702, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 11414 |
| PDB | |
| DBJ | BAA90874 BAD92024 BAE38619 BAF84492 BAG10581 |
| EMBL | CAB51404 CAG30499 CAK54641 CAK54940 |
| GB | AAF01349 AAF32518 AAF99602 AAG09031 AAG09032 |
| REF | NP_001100701 NP_001164822 NP_001178126 NP_001240619 NP_001240620 |
| SP | Q9HBE1 |
| TPG | DAA20504 DAA20505 |
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