BMRB Entry 11336
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11336
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Title: Solution Structure of the RING domain of the human ring finger protein 4
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Yoneyama, M.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Yoneyama, M.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the human ring finger protein 4" . ., .-..
Assembly members:
RING domain, polymer, 69 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RING domain: GSSGSSGTGLRPSGTVSCPI
CMDGYSEIVQNGRLIVSTEC
GHVFCSQCLRDSLKNANTCP
TCRKKINHK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 418 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, RING domain 69 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | GLY | LEU | ||||
| 2 | ARG | PRO | SER | GLY | THR | VAL | SER | CYS | PRO | ILE | ||||
| 3 | CYS | MET | ASP | GLY | TYR | SER | GLU | ILE | VAL | GLN | ||||
| 4 | ASN | GLY | ARG | LEU | ILE | VAL | SER | THR | GLU | CYS | ||||
| 5 | GLY | HIS | VAL | PHE | CYS | SER | GLN | CYS | LEU | ARG | ||||
| 6 | ASP | SER | LEU | LYS | ASN | ALA | ASN | THR | CYS | PRO | ||||
| 7 | THR | CYS | ARG | LYS | LYS | ILE | ASN | HIS | LYS |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RING domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA19122 BAG35433 BAG54620 BAG73691 |
| GB | AAC35248 AAC52022 AAH31935 AAH62024 AAL06715 |
| REF | NP_001171938 NP_002929 NP_062055 XP_003890937 XP_004038387 |
| SP | O88846 P78317 |
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