BMRB Entry 11326
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11326
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Title: Solution structure of the RING domain of the human RING finger protein 146
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the RING domain of the human RING finger protein 146" . ., .-..
Assembly members:
RING domain, polymer, 71 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RING domain: GSSGSSGNTAPSLTVPECAI
CLQTCVHPVSLPCKHVFCYL
CVKGASWLGKRCALCRQEIP
EDFLDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 266 |
| 15N chemical shifts | 56 |
| 1H chemical shifts | 422 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, RING domain 71 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | THR | ALA | ||||
| 2 | PRO | SER | LEU | THR | VAL | PRO | GLU | CYS | ALA | ILE | ||||
| 3 | CYS | LEU | GLN | THR | CYS | VAL | HIS | PRO | VAL | SER | ||||
| 4 | LEU | PRO | CYS | LYS | HIS | VAL | PHE | CYS | TYR | LEU | ||||
| 5 | CYS | VAL | LYS | GLY | ALA | SER | TRP | LEU | GLY | LYS | ||||
| 6 | ARG | CYS | ALA | LEU | CYS | ARG | GLN | GLU | ILE | PRO | ||||
| 7 | GLU | ASP | PHE | LEU | ASP | SER | GLY | PRO | SER | SER | ||||
| 8 | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RING domain, [U-13C; U-15N], 1.34 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB28015 BAB55108 BAB55196 BAB55359 BAE31549 |
| EMBL | CAB66763 CAC85986 CAG38545 CAH89794 CAH90287 |
| GB | AAH08235 AAH50795 AAH83675 AAI02140 AIC52443 |
| REF | NP_001012060 NP_001070448 NP_001103666 NP_001103667 NP_001103668 |
| SP | E1B7X3 Q2PFU6 Q3T139 Q5REL3 Q5XIK5 |
| TPG | DAA20901 DAA26356 |
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