BMRB Entry 11211
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11211
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Title: Solution structure of the third ig-like domain of Myosin-dinding protein C, slow-type
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Kurosaki, C.; Hayashi, F.; Yoshida, M.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Hayashi, F.; Yoshida, M.; Yokoyama, S.. "Solution structure of the third ig-like domain of Myosin-dinding protein C, slow-type" . ., .-..
Assembly members:
IG-like domain, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
IG-like domain: GSSGSSGIMVTKQLEDTTAY
CGERVELECEVSEDDANVKW
FKNGEEIIPGPKSRYRIRVE
GKKHILIIEGATKADAAEYS
VMTTGGQSSAKLSVDLKSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 667 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IG-like domain of Myosin-dinding protein C | 1 |
Entities:
Entity 1, IG-like domain of Myosin-dinding protein C 103 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ILE | MET | VAL | ||||
| 2 | THR | LYS | GLN | LEU | GLU | ASP | THR | THR | ALA | TYR | ||||
| 3 | CYS | GLY | GLU | ARG | VAL | GLU | LEU | GLU | CYS | GLU | ||||
| 4 | VAL | SER | GLU | ASP | ASP | ALA | ASN | VAL | LYS | TRP | ||||
| 5 | PHE | LYS | ASN | GLY | GLU | GLU | ILE | ILE | PRO | GLY | ||||
| 6 | PRO | LYS | SER | ARG | TYR | ARG | ILE | ARG | VAL | GLU | ||||
| 7 | GLY | LYS | LYS | HIS | ILE | LEU | ILE | ILE | GLU | GLY | ||||
| 8 | ALA | THR | LYS | ALA | ASP | ALA | ALA | GLU | TYR | SER | ||||
| 9 | VAL | MET | THR | THR | GLY | GLY | GLN | SER | SER | ALA | ||||
| 10 | LYS | LEU | SER | VAL | ASP | LEU | LYS | SER | GLY | PRO | ||||
| 11 | SER | SER | GLY |
Samples:
sample_1: IG-like domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio F. - processing
NMRView v5.0.4, Johnson B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 900 MHz
Related Database Links:
| PDB |
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