BMRB Entry 11187
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11187
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Title: Solution structure of the 7th PDZ domain of InaD-like protein
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the 7th PDZ domain of InaD-like protein" . ., .-..
Assembly members:
PDZ domain, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGDAFTDQKIRQRYA
DLPGELHIIELEKDKNGLGL
SLAGNKDRSRMSIFVVGINP
EGPAAADGRMRIGDELLEIN
NQILYGRSHQNASAIIKTAP
SKVKLVFIRNEDAVNQMASG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 499 |
| 15N chemical shifts | 121 |
| 1H chemical shifts | 818 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain | 1 |
Entities:
Entity 1, PDZ domain 124 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | ALA | PHE | ||||
| 2 | THR | ASP | GLN | LYS | ILE | ARG | GLN | ARG | TYR | ALA | ||||
| 3 | ASP | LEU | PRO | GLY | GLU | LEU | HIS | ILE | ILE | GLU | ||||
| 4 | LEU | GLU | LYS | ASP | LYS | ASN | GLY | LEU | GLY | LEU | ||||
| 5 | SER | LEU | ALA | GLY | ASN | LYS | ASP | ARG | SER | ARG | ||||
| 6 | MET | SER | ILE | PHE | VAL | VAL | GLY | ILE | ASN | PRO | ||||
| 7 | GLU | GLY | PRO | ALA | ALA | ALA | ASP | GLY | ARG | MET | ||||
| 8 | ARG | ILE | GLY | ASP | GLU | LEU | LEU | GLU | ILE | ASN | ||||
| 9 | ASN | GLN | ILE | LEU | TYR | GLY | ARG | SER | HIS | GLN | ||||
| 10 | ASN | ALA | SER | ALA | ILE | ILE | LYS | THR | ALA | PRO | ||||
| 11 | SER | LYS | VAL | LYS | LEU | VAL | PHE | ILE | ARG | ASN | ||||
| 12 | GLU | ASP | ALA | VAL | ASN | GLN | MET | ALA | SER | GLY | ||||
| 13 | PRO | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.93191, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts