BMRB Entry 10318
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10318
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Title: Solution structure of the PTB domain of KIAA1075 protein from human
Deposition date: 2009-03-11 Original release date: 2010-03-11
Authors: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PTB domain of KIAA1075 protein from human" . ., .-..
Assembly members:
PTB domain, polymer, 160 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis
Entity Sequences (FASTA):
PTB domain: GSSGSSGMSTAADLLRQGAA
CSVLYLTSVETESLTGPQAV
ARASSAALSCSPRPTPAVVH
FKVSAQGITLTDNQRKLFFR
RHYPVNSITFSSTDPQDRRW
TNPDGTTSKIFGFVAKKPGS
PWENVCHLFAELDPDQPAGA
IVTFITKVLLGQRKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 653 |
| 15N chemical shifts | 148 |
| 1H chemical shifts | 1031 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PTB domain | 1 |
Entities:
Entity 1, PTB domain 160 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | SER | THR | |
| 2 | ALA | ALA | ASP | LEU | LEU | ARG | GLN | GLY | ALA | ALA | |
| 3 | CYS | SER | VAL | LEU | TYR | LEU | THR | SER | VAL | GLU | |
| 4 | THR | GLU | SER | LEU | THR | GLY | PRO | GLN | ALA | VAL | |
| 5 | ALA | ARG | ALA | SER | SER | ALA | ALA | LEU | SER | CYS | |
| 6 | SER | PRO | ARG | PRO | THR | PRO | ALA | VAL | VAL | HIS | |
| 7 | PHE | LYS | VAL | SER | ALA | GLN | GLY | ILE | THR | LEU | |
| 8 | THR | ASP | ASN | GLN | ARG | LYS | LEU | PHE | PHE | ARG | |
| 9 | ARG | HIS | TYR | PRO | VAL | ASN | SER | ILE | THR | PHE | |
| 10 | SER | SER | THR | ASP | PRO | GLN | ASP | ARG | ARG | TRP | |
| 11 | THR | ASN | PRO | ASP | GLY | THR | THR | SER | LYS | ILE | |
| 12 | PHE | GLY | PHE | VAL | ALA | LYS | LYS | PRO | GLY | SER | |
| 13 | PRO | TRP | GLU | ASN | VAL | CYS | HIS | LEU | PHE | ALA | |
| 14 | GLU | LEU | ASP | PRO | ASP | GLN | PRO | ALA | GLY | ALA | |
| 15 | ILE | VAL | THR | PHE | ILE | THR | LYS | VAL | LEU | LEU | |
| 16 | GLY | GLN | ARG | LYS | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PTB domain, [U-13C; U-15N], 0.57 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| BMRB | 17364 |
| PDB | |
| DBJ | BAA83027 BAG09960 BAG54765 |
| EMBL | CAB70815 CAH56176 |
| GB | AAH54099 AAI10855 AAI29829 AAI29830 AAI31504 |
| REF | NP_056134 NP_736610 NP_938072 XP_001102202 XP_001790253 |
| SP | Q63HR2 |
| TPG | DAA29990 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts