BMRB Entry 10272
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10272
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Title: Solution structures of the SH3 domain of human KIAA0418
Deposition date: 2008-12-15 Original release date: 2009-12-14
Authors: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human KIAA0418" . ., .-..
Assembly members:
SH3 domain, polymer, 80 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SH3 domain: GSSGSSGATSYMTCSAYQKV
QDSEISFPAGVEVQVLEKQE
SGWWYVRFGELEGWAPSHYL
VLDENEQPDPSGKESGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 480 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 80 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | THR | SER | |
| 2 | TYR | MET | THR | CYS | SER | ALA | TYR | GLN | LYS | VAL | |
| 3 | GLN | ASP | SER | GLU | ILE | SER | PHE | PRO | ALA | GLY | |
| 4 | VAL | GLU | VAL | GLN | VAL | LEU | GLU | LYS | GLN | GLU | |
| 5 | SER | GLY | TRP | TRP | TYR | VAL | ARG | PHE | GLY | GLU | |
| 6 | LEU | GLU | GLY | TRP | ALA | PRO | SER | HIS | TYR | LEU | |
| 7 | VAL | LEU | ASP | GLU | ASN | GLU | GLN | PRO | ASP | PRO | |
| 8 | SER | GLY | LYS | GLU | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9827, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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