BMRB Entry 10165
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10165
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B
Deposition date: 2007-12-28 Original release date: 2009-02-23
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B" . ., .-..
Assembly members:
CBM_21 domain, residues 8-156, polymer, 156 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
CBM_21 domain, residues 8-156: GSSGSSGAESESFVLDFSQP
SADYLDFRNRLQADHVCLEN
CVLKDKAIAGTVKVQNLAFE
KTVKIRMTFDTWKSYTDFPC
QYVKDTYAGSDRDTFSFDIS
LPEKIQSYERMEFAVYYECN
GQTYWDSNRGKNYRIIRAEL
KSTQGMTKPHSGPDLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 689 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 1053 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Protein phosphatase 1, regulatory (Inhibitor) subunit 3B | 1 |
Entities:
Entity 1, Protein phosphatase 1, regulatory (Inhibitor) subunit 3B 156 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | GLU | SER | ||||
| 2 | GLU | SER | PHE | VAL | LEU | ASP | PHE | SER | GLN | PRO | ||||
| 3 | SER | ALA | ASP | TYR | LEU | ASP | PHE | ARG | ASN | ARG | ||||
| 4 | LEU | GLN | ALA | ASP | HIS | VAL | CYS | LEU | GLU | ASN | ||||
| 5 | CYS | VAL | LEU | LYS | ASP | LYS | ALA | ILE | ALA | GLY | ||||
| 6 | THR | VAL | LYS | VAL | GLN | ASN | LEU | ALA | PHE | GLU | ||||
| 7 | LYS | THR | VAL | LYS | ILE | ARG | MET | THR | PHE | ASP | ||||
| 8 | THR | TRP | LYS | SER | TYR | THR | ASP | PHE | PRO | CYS | ||||
| 9 | GLN | TYR | VAL | LYS | ASP | THR | TYR | ALA | GLY | SER | ||||
| 10 | ASP | ARG | ASP | THR | PHE | SER | PHE | ASP | ILE | SER | ||||
| 11 | LEU | PRO | GLU | LYS | ILE | GLN | SER | TYR | GLU | ARG | ||||
| 12 | MET | GLU | PHE | ALA | VAL | TYR | TYR | GLU | CYS | ASN | ||||
| 13 | GLY | GLN | THR | TYR | TRP | ASP | SER | ASN | ARG | GLY | ||||
| 14 | LYS | ASN | TYR | ARG | ILE | ILE | ARG | ALA | GLU | LEU | ||||
| 15 | LYS | SER | THR | GLN | GLY | MET | THR | LYS | PRO | HIS | ||||
| 16 | SER | GLY | PRO | ASP | LEU | GLY |
Samples:
sample_1: filamin domain, [U-13C; U-15N], 1.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20060524, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9823, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAB14811 BAG53215 |
| GB | AAH43388 ADQ33125 AIC57095 EAW65572 EHH63994 |
| REF | NP_001188258 NP_078883 XP_003902491 XP_003902492 XP_004046665 |
| SP | Q86XI6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts