BMRB Entry 10160
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10160
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Title: Solution structure of the 17th filamin domain from human Filamin-B
Deposition date: 2007-12-28 Original release date: 2009-02-20
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 17th filamin domain from human Filamin-B" . ., .-..
Assembly members:
filamin domain, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
filamin domain: GSSGSSGPESPLQFYVNYPN
SGSVSAYGPGLVYGVANKTA
TFTIVTEDAGEGGLDLAIEG
PSKAEISCIDNKDGTCTVTY
LPTLPGDYSILVKYNDKHIP
GSPFTAKITDDSRRC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 470 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 721 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin-B | 1 |
Entities:
Entity 1, Filamin-B 115 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | GLU | SER | ||||
| 2 | PRO | LEU | GLN | PHE | TYR | VAL | ASN | TYR | PRO | ASN | ||||
| 3 | SER | GLY | SER | VAL | SER | ALA | TYR | GLY | PRO | GLY | ||||
| 4 | LEU | VAL | TYR | GLY | VAL | ALA | ASN | LYS | THR | ALA | ||||
| 5 | THR | PHE | THR | ILE | VAL | THR | GLU | ASP | ALA | GLY | ||||
| 6 | GLU | GLY | GLY | LEU | ASP | LEU | ALA | ILE | GLU | GLY | ||||
| 7 | PRO | SER | LYS | ALA | GLU | ILE | SER | CYS | ILE | ASP | ||||
| 8 | ASN | LYS | ASP | GLY | THR | CYS | THR | VAL | THR | TYR | ||||
| 9 | LEU | PRO | THR | LEU | PRO | GLY | ASP | TYR | SER | ILE | ||||
| 10 | LEU | VAL | LYS | TYR | ASN | ASP | LYS | HIS | ILE | PRO | ||||
| 11 | GLY | SER | PRO | PHE | THR | ALA | LYS | ILE | THR | ASP | ||||
| 12 | ASP | SER | ARG | ARG | CYS |
Samples:
sample_1: filamin domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20060524, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9823, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB |
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