BMRB Entry 10148
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10148
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Title: Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2
Deposition date: 2007-12-04 Original release date: 2008-12-10
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2" Not known ., .-..
Assembly members:
zf-C2H2 domains, polymer, 106 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
zf-C2H2 domains: GSSGSSGGRLPSKTKKEFIC
KFCGRHFTKSYNLLIHERTH
TDERPYTCDICHKAFRRQDH
LRDHRYIHSKEKPFKCQECG
KGFCQSRTLAVHKTLHMQTS
SPTAAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 456 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 679 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-C2H2 domains | 1 |
| 2 | ZINC ION No.1 | 2 |
| 3 | ZINC ION No.2 | 2 |
| 4 | ZINC ION No.3 | 2 |
Entities:
Entity 1, zf-C2H2 domains 106 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | ARG | LEU | ||||
| 2 | PRO | SER | LYS | THR | LYS | LYS | GLU | PHE | ILE | CYS | ||||
| 3 | LYS | PHE | CYS | GLY | ARG | HIS | PHE | THR | LYS | SER | ||||
| 4 | TYR | ASN | LEU | LEU | ILE | HIS | GLU | ARG | THR | HIS | ||||
| 5 | THR | ASP | GLU | ARG | PRO | TYR | THR | CYS | ASP | ILE | ||||
| 6 | CYS | HIS | LYS | ALA | PHE | ARG | ARG | GLN | ASP | HIS | ||||
| 7 | LEU | ARG | ASP | HIS | ARG | TYR | ILE | HIS | SER | LYS | ||||
| 8 | GLU | LYS | PRO | PHE | LYS | CYS | GLN | GLU | CYS | GLY | ||||
| 9 | LYS | GLY | PHE | CYS | GLN | SER | ARG | THR | LEU | ALA | ||||
| 10 | VAL | HIS | LYS | THR | LEU | HIS | MET | GLN | THR | SER | ||||
| 11 | SER | PRO | THR | ALA | ALA | SER |
Entity 2, ZINC ION No.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-C2H2 domains, [U-13C; U-15N], 0.86 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20060524, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9823, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAC34859 BAF84180 |
| GB | AAH13504 AAH16936 AAH79211 AAI02144 AAI08580 |
| REF | NP_001012118 NP_001029500 NP_001089872 NP_001090353 NP_001163815 |
| SP | Q0IHB8 Q32NK7 Q3T135 Q6AY34 |
| TPG | DAA22584 |
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