BMRB Entry 10142
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10142
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B
Deposition date: 2007-12-04 Original release date: 2008-12-10
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B" Not known ., .-..
Assembly members:
filamin domain, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
filamin domain: GSSGSSGRVKESITRTSRAP
SVATVGSICDLNLKIPEINS
SDMSAHVTSPSGRVTEAEIV
PMGKNSHCVRFVPQEMGVHT
VSVKYRGQHVTGSPFQFTVG
PLGEGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 670 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin-B | 1 |
Entities:
Entity 1, Filamin-B 106 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | VAL | LYS | ||||
| 2 | GLU | SER | ILE | THR | ARG | THR | SER | ARG | ALA | PRO | ||||
| 3 | SER | VAL | ALA | THR | VAL | GLY | SER | ILE | CYS | ASP | ||||
| 4 | LEU | ASN | LEU | LYS | ILE | PRO | GLU | ILE | ASN | SER | ||||
| 5 | SER | ASP | MET | SER | ALA | HIS | VAL | THR | SER | PRO | ||||
| 6 | SER | GLY | ARG | VAL | THR | GLU | ALA | GLU | ILE | VAL | ||||
| 7 | PRO | MET | GLY | LYS | ASN | SER | HIS | CYS | VAL | ARG | ||||
| 8 | PHE | VAL | PRO | GLN | GLU | MET | GLY | VAL | HIS | THR | ||||
| 9 | VAL | SER | VAL | LYS | TYR | ARG | GLY | GLN | HIS | VAL | ||||
| 10 | THR | GLY | SER | PRO | PHE | GLN | PHE | THR | VAL | GLY | ||||
| 11 | PRO | LEU | GLY | GLU | GLY | GLY |
Samples:
sample_1: filamin domain, [U-13C; U-15N], 1.53 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20060524, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts