BMRB Entry 10138
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10138
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8 PubMed: 17616598
Deposition date: 2007-06-04 Original release date: 2008-06-12
Authors: Suzuki, S.; Matsumoto, E.; Tatsuguchi, A.; Kawazoe, M.; Kaminishi, T.; Takemoto, C.; Shirouzu, M.; Muto, Y.; Yokoyama, S.
Citation: Suzuki, S.; Tatsuguchi, A.; Matsumoto, E.; Kawazoe, M.; Kaminishi, T.; Shirouzu, M.; Muto, Y.; Takemoto, C.; Yokoyama, S.. "Structural characterization of the ribosome maturation protein, RimM" J. Bacteriol. 189, .-6397 (6406).
Assembly members:
RIEF fold, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 300852 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
RIEF fold: MRLVEIGRFGAPYALKGGLR
FRGEPVVLHLERVYVEGHGW
RAIEDLYRVGEELVVHLAGV
TDRTLAEALVGLRVYAEVAD
LPPLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 388 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 623 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Probable 16S rRNA-processing protein rimM | 1 |
Entities:
Entity 1, Probable 16S rRNA-processing protein rimM 85 residues - Formula weight is not available
| 1 | MET | ARG | LEU | VAL | GLU | ILE | GLY | ARG | PHE | GLY | ||||
| 2 | ALA | PRO | TYR | ALA | LEU | LYS | GLY | GLY | LEU | ARG | ||||
| 3 | PHE | ARG | GLY | GLU | PRO | VAL | VAL | LEU | HIS | LEU | ||||
| 4 | GLU | ARG | VAL | TYR | VAL | GLU | GLY | HIS | GLY | TRP | ||||
| 5 | ARG | ALA | ILE | GLU | ASP | LEU | TYR | ARG | VAL | GLY | ||||
| 6 | GLU | GLU | LEU | VAL | VAL | HIS | LEU | ALA | GLY | VAL | ||||
| 7 | THR | ASP | ARG | THR | LEU | ALA | GLU | ALA | LEU | VAL | ||||
| 8 | GLY | LEU | ARG | VAL | TYR | ALA | GLU | VAL | ALA | ASP | ||||
| 9 | LEU | PRO | PRO | LEU | GLU |
Samples:
sample_1: RIEF fold, [U-13C; U-15N], 1.2 mM; sodium phosphate 20 mM; D2O 10%; H2O 90%
condition_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
Olivia v1.10.5, Yokochi, M. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
SPARKY v3.110, Goddard, T.D., Kneller, D.G. - data analysis
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 10139 10140 |
| PDB | |
| DBJ | BAD70856 |
| GB | AAS81019 AEG33454 AFH38925 EIA39096 |
| REF | WP_008632437 WP_011228392 YP_144299 |
| SP | Q5SJH5 Q72JU7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts