BMRB Entry 10128
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10128
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Title: Solution structure of the second FNIII domain of DSCAML1 protein
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Qin, X.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second FNIII domain of DSCAML1 protein" Not known ., .-..
Assembly members:
FNIII domain, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
FNIII domain: GSSGSSGISTEEAAPDGPPM
DVTLQPVTSQSIQVTWKAPK
KELQNGVIRGYQIGYRENSP
GSNGQYSIVEMKATGDSEVY
TLDNLKKFAQYGVVVQAFNR
AGTGPSSSEINATTLESGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 503 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 799 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | second FNIII domain of DSCAML1 protein | 1 |
Entities:
Entity 1, second FNIII domain of DSCAML1 protein 122 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ILE | SER | THR | ||||
| 2 | GLU | GLU | ALA | ALA | PRO | ASP | GLY | PRO | PRO | MET | ||||
| 3 | ASP | VAL | THR | LEU | GLN | PRO | VAL | THR | SER | GLN | ||||
| 4 | SER | ILE | GLN | VAL | THR | TRP | LYS | ALA | PRO | LYS | ||||
| 5 | LYS | GLU | LEU | GLN | ASN | GLY | VAL | ILE | ARG | GLY | ||||
| 6 | TYR | GLN | ILE | GLY | TYR | ARG | GLU | ASN | SER | PRO | ||||
| 7 | GLY | SER | ASN | GLY | GLN | TYR | SER | ILE | VAL | GLU | ||||
| 8 | MET | LYS | ALA | THR | GLY | ASP | SER | GLU | VAL | TYR | ||||
| 9 | THR | LEU | ASP | ASN | LEU | LYS | LYS | PHE | ALA | GLN | ||||
| 10 | TYR | GLY | VAL | VAL | VAL | GLN | ALA | PHE | ASN | ARG | ||||
| 11 | ALA | GLY | THR | GLY | PRO | SER | SER | SER | GLU | ILE | ||||
| 12 | ASN | ALA | THR | THR | LEU | GLU | SER | GLY | PRO | SER | ||||
| 13 | SER | GLY |
Samples:
sample_1: FNIII domain, [U-13C; U-15N], 1.05 mM; PiNa 20 mM; NaCl 100 mM; d10-DTT 1.0 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.854, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| GB | EDL25653 |
| REF | XP_008567853 XP_009635232 XP_009915740 XP_010117466 XP_010180789 |
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