BMRB Entry 10120
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10120
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Title: Solution structure of immunoglobulin like domain of mouse nuclear lamin
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of immunoglobulin like domain of mouse nuclear lamin" Not known ., .-..
Assembly members:
C-terminal immunoglobulin like domain, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
C-terminal immunoglobulin like domain: GSSGSSGQSQGGGSVTKKRK
LESSESRSSFSQHARTSGRV
AVEEVDEEGKFVRLRNKSNE
DQSMGNWQIRRQNGDDPLMT
YRFPPKFTLKAGQVVTIWAS
GAGATHSPPTDLVWKAQNTW
GCGSSLRTALINSTGEEVAM
RKLVRSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 568 |
| 15N chemical shifts | 158 |
| 1H chemical shifts | 922 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lamin A | 1 |
Entities:
Entity 1, Lamin A 151 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | SER | GLN | ||||
| 2 | GLY | GLY | GLY | SER | VAL | THR | LYS | LYS | ARG | LYS | ||||
| 3 | LEU | GLU | SER | SER | GLU | SER | ARG | SER | SER | PHE | ||||
| 4 | SER | GLN | HIS | ALA | ARG | THR | SER | GLY | ARG | VAL | ||||
| 5 | ALA | VAL | GLU | GLU | VAL | ASP | GLU | GLU | GLY | LYS | ||||
| 6 | PHE | VAL | ARG | LEU | ARG | ASN | LYS | SER | ASN | GLU | ||||
| 7 | ASP | GLN | SER | MET | GLY | ASN | TRP | GLN | ILE | ARG | ||||
| 8 | ARG | GLN | ASN | GLY | ASP | ASP | PRO | LEU | MET | THR | ||||
| 9 | TYR | ARG | PHE | PRO | PRO | LYS | PHE | THR | LEU | LYS | ||||
| 10 | ALA | GLY | GLN | VAL | VAL | THR | ILE | TRP | ALA | SER | ||||
| 11 | GLY | ALA | GLY | ALA | THR | HIS | SER | PRO | PRO | THR | ||||
| 12 | ASP | LEU | VAL | TRP | LYS | ALA | GLN | ASN | THR | TRP | ||||
| 13 | GLY | CYS | GLY | SER | SER | LEU | ARG | THR | ALA | LEU | ||||
| 14 | ILE | ASN | SER | THR | GLY | GLU | GLU | VAL | ALA | MET | ||||
| 15 | ARG | LYS | LEU | VAL | ARG | SER | GLY | PRO | SER | SER | ||||
| 16 | GLY |
Samples:
sample_1: lamin, [U-13C; U-15N], 0.88 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
sample_2: lamin, [U-13C; U-15N], 0.70 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; D2O 100%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY 1 | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY 2 | sample_2 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v2002045, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.816, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA02476 BAA03578 BAA08569 BAA08570 BAA08571 |
| GB | AAH15302 AAH94020 ABI16251 ABI16252 EDL15275 |
| REF | NP_001002011 NP_001104572 NP_062263 XP_003502967 XP_005080182 |
| SP | P48678 |
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