BMRB Entry 10088
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10088
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Title: Solution structure of the second PDZ domain of human scribble (KIAA0147 protein)
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second PDZ domain of human scribble (KIAA0147 protein)" . ., .-..
Assembly members:
PDZ domain, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PDZ domain: GSSGSSGRHVACLARSERGL
GFSIAGGKGSTPYRAGDAGI
FVSRIAEGGAAHRAGTLQVG
DRVLSINGVDVTEARHDHAV
SLLTAASPTIALLLEREAGS
GPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 392 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 617 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIAA0147 protein | 1 |
Entities:
Entity 1, KIAA0147 protein 105 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | HIS | VAL | ||||
| 2 | ALA | CYS | LEU | ALA | ARG | SER | GLU | ARG | GLY | LEU | ||||
| 3 | GLY | PHE | SER | ILE | ALA | GLY | GLY | LYS | GLY | SER | ||||
| 4 | THR | PRO | TYR | ARG | ALA | GLY | ASP | ALA | GLY | ILE | ||||
| 5 | PHE | VAL | SER | ARG | ILE | ALA | GLU | GLY | GLY | ALA | ||||
| 6 | ALA | HIS | ARG | ALA | GLY | THR | LEU | GLN | VAL | GLY | ||||
| 7 | ASP | ARG | VAL | LEU | SER | ILE | ASN | GLY | VAL | ASP | ||||
| 8 | VAL | THR | GLU | ALA | ARG | HIS | ASP | HIS | ALA | VAL | ||||
| 9 | SER | LEU | LEU | THR | ALA | ALA | SER | PRO | THR | ILE | ||||
| 10 | ALA | LEU | LEU | LEU | GLU | ARG | GLU | ALA | GLY | SER | ||||
| 11 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.1 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8994, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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